Exogenous, basal, and flow-induced nitric oxide production and endothelial cell proliferation

Journal of Cellular Physiology
K J GoochJ A Frangos

Abstract

The role of nitric oxide (NO) from endogenous and exogenous sources in regulating large vessel and microvascular endothelial cell proliferation was investigated. Exogenous NO liberated from five different chemical donors inhibited bovine aortic, bovine retinal microvascular, and human umbilical vein endothelial cell proliferation in a dose-dependent manner as determined by 3H-thymidine incorporation. The potency of the donors varied as a function of the donors' half-lives. Donors with half-lives greater than 30 min were more effective than donors with significantly shorter half-lives. Coincubation of endothelial cells with 0.4 mM deoxyadenosine and 0.4 mM deoxyguanosine reduced the percentage of inhibition due to an NO donor. These data are consistent with a ribonucleotide reductase-dependent mechanism of inhibition. Inhibition of basal NO production with four different inhibitors of nitric oxide synthase (NOS) did not modify proliferation. Laminar flow with a wall shear stress of 22 dyn/cm2 inhibited the proliferation of subconfluent bovine aortic endothelial cells. The addition of a NOS inhibitor did not abrogate the flow-induced inhibition of proliferation, suggesting that flow-stimulated release of NO from endothelial cells...Continue Reading

References

Oct 1, 1991·The Journal of Experimental Medicine·N S KwonC F Nathan
Oct 1, 1988·Analytical Biochemistry·Y J KimA J Grodzinsky
Jan 1, 1988·Annual Review of Biochemistry·P Reichard
Nov 1, 1994·The American Journal of Physiology·J F ArnalD G Harrison
Sep 15, 1994·Biochemical and Biophysical Research Communications·W YangA Kamiya
Mar 1, 1994·The American Journal of Physiology·M J Kuchan, J A Frangos
May 14, 1993·Biochemical and Biophysical Research Communications·M ZicheF Ledda

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Citations

Apr 19, 2011·Angiogenesis·Anna Maria CuratolaKaren D Hendricks-Munoz
Aug 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B FermorF Guilak
Jun 15, 2010·Cardiovascular Research·John M Tarbell
Apr 7, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Christina M WarboysPeter D Weinberg
Dec 21, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Limary M Cancel, John M Tarbell
Jun 17, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Eleni MetaxaJohn Kolega
Oct 16, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Jianxin Sun, James K Liao
Dec 19, 2007·Pharmacogenomics·Glen E CookePhilip F Binkley
Mar 26, 2003·Annual Review of Biomedical Engineering·John M Tarbell
Jan 25, 2017·Expert Opinion on Drug Delivery·Mahmoud A ElnaggarYoon Ki Joung
Aug 13, 2004·Vascular and Endovascular Surgery·Joseph L UnthankMichael C Dalsing
Oct 11, 2017·Journal of Tissue Engineering·Tarek M BedairDong Keun Han
Jul 9, 2005·Journal of Biomaterials Science. Polymer Edition·Kristyn S Bohl MastersJennifer L West
Jan 15, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·Y S ChangJ M Tarbell
May 18, 2006·The FEBS Journal·Antonio Villalobo
Aug 13, 2008·Journal of Burn Care & Research : Official Publication of the American Burn Association·Haifeng ZhuFerid Murad
Jan 27, 2006·Journal of the American Society of Nephrology : JASN·Takahiko NakagawaRichard J Johnson
Jan 20, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Z L SzomorG A C Murrell
Aug 28, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Louise OstergaardUlf Simonsen
Feb 13, 2015·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Qing-Hui TangZheng-Xiang Liu
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·H T YangR L Terjung
Jul 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·R S MarinosC J Meininger
Nov 28, 2008·American Journal of Physiology. Renal Physiology·Takahiro NakayamaTakahiko Nakagawa
Feb 13, 2003·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·J E WoodellR H Hilderman
Jun 27, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Nasim Salehi-NikJenneke Klein-Nulend
Apr 26, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·L PelletierP Charbord
Jul 27, 2000·Endothelium : Journal of Endothelial Cell Research·S ZöllnerG M Rubanyi
Aug 13, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sujeong JangHeonyong Park
Jan 2, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Gurneet S SanghaAlisa M Clyne
Mar 16, 2021·Frontiers in Physiology·Laia Gifre-Renom, Elizabeth A V Jones
Dec 19, 2002·Vascular Pharmacology·Guilia RussoJoseph Loscalzo
Oct 19, 2006·The International Journal of Biochemistry & Cell Biology·Zhen-Zhong Zheng, Zheng-Xiang Liu

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