Exogenous collagen cross-linking reduces scleral permeability: modeling the effects of age-related cross-link accumulation

Investigative Ophthalmology & Visual Science
Jay M StewartMonique L Trinidad

Abstract

To investigate the relationship between scleral permeability and nonenzymatic cross-link density. Scleral discs 18 mm in diameter were dissected from the medial and lateral equatorial regions of 60 cadaveric porcine eyes. Samples were incubated for 24 hours with control solution or methylglyoxal at concentrations of 0.001%, 0.01%, 0.10%, and 1.00%. Nonenzymatic cross-link density in treated and control groups was quantified with the use of papain digest and fluorescence spectrophotometry. Treated scleral discs were mounted in a customized Ussing-type chamber connected to vertical tubing, and specific hydraulic conductivity was determined according to the descent of a column of degassed saline at room temperature. Permeability to diffusion of fluorescein in a static chamber was determined for another set of treated scleral samples. Methylglyoxal treatment effectively increased nonenzymatic cross-link content, as indicated by the average fluorescence for each group. Specific hydraulic conductivity (m(2)) was reduced with increasing cross-link density. Similarly, the permeability coefficient for the fluorescein solute consistently decreased with increasing methylglyoxal concentration, indicating diffusion impedance from the treatm...Continue Reading

Citations

Jul 12, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Catherine F WhittingtonSherry L Voytik-Harbin
Apr 16, 2013·The Ocular Surface·Frederik Raiskup, Eberhard Spoerl
Nov 28, 2012·Journal of the Mechanical Behavior of Biomedical Materials·Brendan GeraghtyAhmed Elsheikh
Aug 14, 2012·Experimental Eye Research·Fergus F WongJay M Stewart
Mar 12, 2010·Experimental Eye Research·Ahmed ElsheikhPaolo Rama
Feb 18, 2010·Survey of Ophthalmology·Mohammed ElagouzTimothy L Jackson
Feb 15, 2014·BioMed Research International·Yali ZhangGuoying Mu
Sep 25, 2012·Current Opinion in Pharmacology·Nicholas G Strouthidis, Michael J A Girard
Nov 15, 2017·Retina·Nopasak PhasukkijwatanaDavid Sarraf
Sep 23, 2010·Current Eye Research·G BlatsiosS P Gartaganis
Apr 7, 2017·Journal of the Royal Society, Interface·Ian C CampbellC Ross Ethier
Jan 21, 2021·Journal of the Royal Society, Interface·Dillon M BrownC Ross Ethier

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