Exogenous expression of human granulocyte colony-stimulating factor receptor in a B-lineage acute lymphoblastic leukemia cell line: a possible model for mixed lineage leukemia

Leukemia Research
S S el-SonbatyI Matsuda

Abstract

We report evidence that the granulocyte colony-stimulating factor (G-CSF) receptor is present and may be functioning on blast cells from some patients with myeloid surface antigen positive (My+) acute lymphoblastic leukemia (ALL). In the present study, a human G-CSF receptor expression plasmid was transfected into a newly established B-lineage ALL cell line 'Tanoue' and its subclone 'ST' by lipofection to investigate whether expression of the G-CSF receptor and G-CSF stimulation would induce myeloid characteristics on myeloid surface antigen negative (My-) ALL cells. The G-CSF receptor became detectable on the transfected cells (GR-Tanoue and GR-ST), with dissociation constant values of 50-130 pmol/l, and maximal binding sites (Bmax of 77-6100 sites/cell on receptor binding assays. Short term culture with recombinant human G-CSF induced myeloid differentiation (a two to three-fold increase in CD33 and CD15 expression), and a moderate 3H-thymidine uptake (stimulation index, 1.75) only in the GR-ST clone no. 15 which expressed a high number of G-CSF receptors (Bmax, 6100 sites/cell). Our data show that (a) exogenous expression of the G-CSF receptor and G-CSF stimulation can induce myeloid characteristics on ALL cells; and (b) in ...Continue Reading

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Citations

Apr 22, 1997·International Journal of Cancer. Journal International Du Cancer·M HigashiyamaM Miyake
Feb 9, 2012·Current Opinion in Immunology·Mark P ChaoRavindra Majeti
Oct 15, 2009·Hematology/oncology Clinics of North America·Dario Campana
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Dec 5, 2006·Ageing Research Reviews·Cosimo TortorellaSalvatore Antonaci
Mar 28, 2016·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Atsushi MizumaShunya Takizawa

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