Exogenous expression of UHRF1 promotes proliferation and metastasis of breast cancer cells

Oncology Reports
Xinli LiSaijun Fan

Abstract

In the present study, we investigated the role of UHRF1 (ubiquitin-like protein containing PHD and RING finger domains 1) in proliferation, invasion and migration of breast cancer cells, and the potential mechanisms were also explored. Cell proliferation was examined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay; cell cycle distribution and apoptosis were evaluated using flow cytometry; protein expression was determined by western blotting; angiogenesis of xenografts was assessed by microvessel density (MVD); cell invasion was measured using transwell chamber; cell migration was determined by wound scratching assay. Our results demonstrated that UHRF1 transfection conferred serum independence to MDA-MB-231 cells, G1 phase shortage and apoptosis suppression, accompanied with an increased expression of cyclin D1 and decreased expression of Bax. Significant pro-invasion and pro-migration activity was observed, with no obvious effect on the expression of PTEN and maspin. Co-expression of the UHRF1/PTEN or UHRF1/maspin degraded the role of UHRF1 in regulating invasion and migration. UHRF1 induced growth of MDA-MB-231 cells by promoting tumor vessel formation in vivo. In conclusion, UHRF1 promoted the p...Continue Reading

Citations

Nov 3, 2012·PloS One·Elizabeth IornsMarc Lippman
Jan 16, 2016·Proceedings of the National Academy of Sciences of the United States of America·Kenzui TaniueTetsu Akiyama
Feb 21, 2016·Journal of Digestive Diseases·Azam SoleimaniShiva Irani
May 13, 2020·Cancer Metastasis Reviews·Alejandra I FerrerPranela Rameshwar
Jun 28, 2019·Frontiers in Oncology·Niaz Mahmood, Shafaat A Rabbani

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