Exogenous oxytocin modulates human myometrial microRNAs

American Journal of Obstetrics and Gynecology
Joanna R CookVasso Terzidou

Abstract

MicroRNAs (miRNAs) play a modulatory role in pathways that lead to labor onset, although oxytocin is known to modulate gene expression within the myometrium. We aimed to identify miRNAs whose expression is regulated by oxytocin in pregnant human myometrium. Myometrial miRNA expression profiles were compared between samples collected from women at term before the onset of labor (no labor; n = 8) and after labor onset after early exogenous oxytocin treatment (n = 8). Multivariate modelling was used to assess differences in miRNA profiles. Biologic validation was undertaken on 3 independent patient cohorts (no labor, n = 10; labor induced with oxytocin, n = 8; and spontaneous labor with no oxytocin treatment, n = 10). In vitro studies that used primary myocytes were undertaken to assess target miRNA expression after oxytocin treatment. Target genes of candidate miRNAs were identified in silico and cross-referenced with genes that are known to be associated with labor or expressed in myometrium. In total, 1309 miRNAs were analyzed by microarray, of which 494 were detected in human myometrium. Multivariate modeling identified 12 target miRNAs the differential expression of which was most responsible for the observed separation of th...Continue Reading

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Citations

Jan 26, 2017·Molecular and Cellular Endocrinology·Sung Hye KimVasso Terzidou
Jun 14, 2018·Physiological Reviews·Benjamin Jurek, Inga D Neumann
Jun 28, 2020·Biology of Reproduction·Joan C WijayaBill Kalionis
Mar 24, 2018·Molecular Medicine Reports·Sharzehan Mohamad Ayub KhanWei Cun See Too
Feb 13, 2021·Archives of Gynecology and Obstetrics·Rodolfo R FavaroTelma M T Zorn
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Zhenguo YangQingyun Chen
Mar 19, 2021·Epigenetics : Official Journal of the DNA Methylation Society·Caitlin G HoweCarrie V Breton
Apr 22, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qiuran XuKangsheng Tu
Aug 10, 2021·Journal of Pediatric Gastroenterology and Nutrition·Einat Gutman-IdoRegina Golan-Gerstl

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