Exogenous phytohormone-independent growth and regeneration of tobacco plants transgenic for the 6b gene of Agrobacterium tumefaciens AKE10

Plant Physiology
Hiroetsu Wabiko, M Minemura

Abstract

The 6b gene of Agrobacterium tumefaciens AKE10 (AK-6b) induces crown gall tumors on certain plants but so far there have been no reports of the gene being able to induce tumors on culture medium. We cloned T-DNA segments containing the 6b gene but lacking the auxin and cytokinin biosynthesis genes from A. tumefaciens AKE10. Tobacco (Nicotiana tabacum) leaf discs infected with A. tumefaciens LBA4404 carrying the clones produced shooty calli on hormone-free Murashige-Skoog medium. The relevant T-DNA segment was integrated into plant DNA as determined by Southern hybridization. Some of these immature shoots spontaneously developed into mature shoots, of which several leaves displayed morphological abnormalities. When leaf discs of these mature plants were placed onto the same medium numerous shoots developed from the wounding sites, indicating that the transgenic plants possessed a high regenerative potential. Northern blot and reverse transcriptase-polymerase chain reaction analyses showed a large accumulation of the AK-6b transcripts in the shooty calli, but only a limited degree in mature plants, demonstrating that AK-6b expression is regulated in plants and essential for the early stages of regeneration. Cytokinin levels in th...Continue Reading

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Citations

Sep 19, 2002·Molecular Plant-microbe Interactions : MPMI·Marie UmberLéon Otten
Sep 25, 2007·The Plant Cell·Shinji TerakuraYasunori Machida
Mar 30, 2001·Molecular Plant-microbe Interactions : MPMI·L Otten, A Helfer
Dec 24, 2003·The Plant Journal : for Cell and Molecular Biology·Louis GrémillonLéon Otten
Apr 14, 2004·The Plant Journal : for Cell and Molecular Biology·Hiroshi MoriuchiNobukazu Tanaka
Mar 2, 2006·The Plant Journal : for Cell and Molecular Biology·Bernadette ClémentLéon Otten
Feb 20, 2015·Journal of Plant Research·Masaki Ito, Yasunori Machida
Sep 10, 2004·Plant & Cell Physiology·Makiko UdagawaKunihiko Syono
Apr 8, 2021·Plant Molecular Biology·Léon Otten

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