Exogenous proline stimulates type I collagen and HIF-1α expression and the process is attenuated by glutamine in human skin fibroblasts

Molecular and Cellular Biochemistry
Lukasz SzokaJerzy Palka

Abstract

Abundance of proline (Pro) in collagen molecule led us to investigate whether Pro supply affects collagen biosynthesis in human skin fibroblasts. Treatment of the cells with milimolar concentrations (5 and 10 mM) of Pro for 24 and 48 h contributed to increase in α1 subunit of collagen type I (COL1A1) expression in both cells and culture medium. However, the effect was more pronounced in glutamine-free medium. In such condition, Pro induced collagen expression by about twofold in the cells, while in the medium only by about 30% during 24 h incubation, compared to control. In the presence of glutamine (Gln), exogenous Pro stimulated intracellular collagen expression only by about 30% during 24 h of fibroblasts incubation, and it was not accompanied by adequate increase of collagen secretion into medium. Gln alone stimulated the processes by about 2-3 fold during the course of the experiment. Pro-dependent increase in collagen expression in Gln-free medium was accompanied by increase in prolidase activity and expression of pAkt. In both Gln-free medium and Gln-supplemented medium, Pro induced expression of p53 and HIF-1α. The data suggest that availability of Gln, as a substrate for Pro biosynthesis, determine the utilization of e...Continue Reading

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Citations

Nov 20, 2019·Cellular and Molecular Life Sciences : CMLS·Ewa KarnaJerzy A Palka
May 28, 2020·Biology·Marta SpodenkiewiczAnne-Sophie Lèbre
Feb 5, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Meike HasenzahlStephan Reichl
Aug 31, 2021·The International Journal of Biochemistry & Cell Biology·Chuin Ying UngTanya J Shaw

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