Exogenous S-nitrosoglutathione attenuates inflammatory response and intestinal epithelial barrier injury in endotoxemic rats

The Journal of Trauma and Acute Care Surgery
Zhi-Feng LiJianguo Li

Abstract

Gut barrier injury in sepsis is a major contributor to distant organ dysfunction and bad clinical outcomes. Enteric glia-derived S-nitrosoglutathione (GSNO) has been recognized as a novel modulator of gut barrier integrity. In this study, we tested the potential therapeutic effect and mechanism of exogenous GSNO on endotoxin-induced inflammatory response and intestinal barrier injury in a rat model of endotoxemia. Male Sprague-Dawley rats were randomly assigned to four groups as follows: control (saline only), GSNO, lipopolysaccharide (LPS), and LPS + GSNO. Femoral venous injection of LPS (10 mg/kg) or saline was followed by GSNO (1 mg/kg) or saline injection 15 minutes later. Distal ileum tissues and blood were harvested after 3 hours of LPS/saline injection. The intestinal barrier function was measured histologically and by intestinal permeability to fluorescein isothiocyanate dextran. The ultrastructural change of the epithelial tight junction was observed using transmission electron microscope, and the expression level of tight junction protein ZO-1 was analyzed using immunofluorescence and Western blot. Systemic and intestinal inflammation was measured by analyzing the tumor necrosis factor and interleukin 1β levels in pla...Continue Reading

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Citations

Jan 1, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Aaron K Chow, Brian D Gulbransen
Jun 14, 2018·Physiology·Nathalie Vergnolle, Carla Cirillo
Nov 23, 2018·The Journal of Surgical Research·Yu WangRong Zhang
May 28, 2021·Clinical and Experimental Pharmacology & Physiology·Shrouk S ElzokmMahmoud M El-Mas

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