Exogenously injected nuclear import factor p10/NTF2 inhibits signal-mediated nuclear import and export of proteins in living cells

FEBS Letters
T TachibanaY Yoneda

Abstract

p10/NTF2 is a cytosolic factor which is required for the translocation step in nuclear protein import in an in vitro assay with digitonin-permeabilized cells. To study the functional roles of p10/NTF2 on protein transport between the nucleus and cytoplasm in living cells, recombinant p10/NTF2 was micro-injected into cultured mammalian cells. Cytoplasmically injected p10/NTF2 strongly inhibited the nuclear import of co-injected NLS-containing substrates in a dose-dependent manner but had no effect on the diffusive import of small non-nuclear proteins. Moreover, when injected into the cell nucleus, p10/NTF2 inhibited the nuclear export of NES-containing substrates. The results suggest that the nuclear import factor p10/NTF2 may also be involved in the nuclear export of proteins and that the protein transport efficiency between the nucleus and cytoplasm may be regulated by the intracellular level of p10/NTF2.

References

May 25, 1988·Nucleic Acids Research·U GrundmannH A Küpper
Apr 21, 1995·Cell·E Izaurralde, I W Mattaj
Jun 1, 1995·Current Opinion in Cell Biology·F Melchior, L Gerace
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·J Moroianu, G Blobel
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·G SchlenstedtP A Silver
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A RaduM S Moore
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·J MoroianuA Radu
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·M S Moore, G Blobel
Jan 1, 1995·Methods in Enzymology·F MelchiorL Gerace
Apr 5, 1996·Science·U Nehrbass, G Blobel
Jan 1, 1996·Trends in Microbiology·Y Yoneda

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Citations

Jun 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D A JansM H Lam
Oct 13, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y Yoneda
Oct 19, 2000·The Journal of Cell Biology·C M LaneM S Moore
Apr 10, 1999·Molecular Biology of the Cell·F YokoyaY Yoneda
Feb 2, 2006·Molecular and Cellular Biology·Akira KurisakiAristidis Moustakas
Aug 27, 2005·BMC Cell Biology·Svetlana MinakhinaRuth Steward
Dec 22, 2005·Experimental Cell Research·Yoshinari YasudaYoshihiro Yoneda
Apr 20, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hideki YamasakiYoshihiro Yoneda
Jul 31, 1998·Experimental Cell Research·N KudoM Yoshida
Oct 6, 1997·Science·B Ossareh-NazariC Dargemont
Oct 7, 2005·The Journal of Biological Chemistry·Yoshiteru MiyauchiKeiichi Ozono
Apr 20, 2005·Journal of Cellular Biochemistry·Wei HuDavid A Jans
Nov 24, 1999·Molecular and Cellular Biology·B E BlackB M Paschal
Apr 10, 1999·Plant Physiology·H M Smith, N V Raikhel

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