PMID: 8975703Dec 15, 1996Paper

Exon mapping by fiber-FISH or LR-PCR

Genomics
R J FlorijnA K Raap

Abstract

In this study we systematically assessed the sensitivity limits of fiber-FISH in model experiments. Exonic fragments and cDNAs with exon sizes of >/=200 bp could be mapped on their cognate cosmid. This positional fiber-FISH mapping was validated by long-range PCR. It is expected that these two independent mapping approaches will help to refine current available gene maps and show their applicability in fine mapping of sequence-tagged sites or expressed sequence tags. Also, they will be useful in resolving gene structures by mapping exon and intron locations.

Citations

Aug 26, 1998·Molecular Pathology : MP·J J WatersC P Gould
Mar 5, 2004·The Plant Journal : for Cell and Molecular Biology·M ValárikJ Dolezel
Sep 18, 2001·Methods : a Companion to Methods in Enzymology·M P van de Corput, F G Grosveld
Jan 25, 2002·American Journal of Medical Genetics·Caterina CintiNadir Mario Maraldi
May 23, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·F M van de RijkeA K Raap
Jul 18, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H U Weier
Jul 17, 1999·Trends in Plant Science· Hans de Jong JP Zabel
Sep 12, 2002·Fungal Genetics and Biology : FG & B·Dai TsuchiyaMasatoki Taga

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