Exon Skipping of FcεRIβ for Allergic Diseases

Methods in Molecular Biology
Greer K Arthur, Glenn Cruse

Abstract

Mast cells are key effector cells in allergic inflammation and consequently are ideal targets for new therapeutics. The high-affinity IgE receptor complex, FcεRI, plays a critical role in mast cell and basophil activation by allergens to drive the immediate allergic inflammatory response. The β subunit of FcεRI is critical for trafficking the FcεRI complex to the cell membrane and amplifies the FcεRI signaling cascade. We have utilized splice switching antisense oligonucleotides to force expression of a truncated isoform of FcεRIβ, which we have shown does not associate with the FcεRI complex. This approach eliminates surface FcεRI expression in mast cells by targeting protein-protein interactions. Exon skipping has several therapeutic applications, and our findings demonstrate a novel application to alter receptor trafficking and dampen allergic inflammation. Here, we describe the methods of exon skipping in mast cells and the assays used to examine the responses of mast cells in vitro and in vivo.

Citations

Jun 3, 2020·Gene Therapy·Kasia Dzierlega, Toshifumi Yokota

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.

Anaphylaxis

Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Glenn CruseDean D Metcalfe
FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Glenn CrusePeter Bradding
Frontiers in Immunology
Chisei RaYoshimichi Okayama
Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
P KorošecP J Turner
© 2022 Meta ULC. All rights reserved