Exonuclease III-assisted cascade signal amplification strategy for label-free and ultrasensitive chemiluminescence detection of DNA

Analytical Chemistry
Yuan Gao, Baoxin Li

Abstract

Detection of ultralow concentrations of specific nucleic acid sequences is a central challenge in the early diagnosis of genetic diseases and biodefense applications. Herein, we report a simple and homogeneous chemiluminescence (CL) method for ultrasensitive DNA detection. It is based on the exonuclease III (Exo III)-assisted cascade signal amplification and the catalytic effect of G-quadruplex-hemin DNAzyme on the luminol-H2O2 CL system. A quadruplex-forming DNA probe hybridizes a hairpin DNA probe to construct a duplex DNA probe as recognition element. Upon sensing of target DNA, the recognition of target DNA and the duplex DNA probe triggers the Exo III cleavage process, accompanied by releasing target DNA and generating a new secondary target DNA fragment. The released target DNA and the secondary target DNA are recycled. Simultaneously, numerous quadruplex-forming sequences are liberated and bind hemin to yield G-quadruplex-hemin DNAzyme, which subsequently catalyze the luminol-H2O2 reaction to produce strong CL emission. This method exhibited a high sensitivity toward target DNA with a detection limit of 8 fM, which was about 100 times lower than that of the reported DNAzyme-based colorimetric system for DNA detection wit...Continue Reading

References

Dec 17, 2015·ACS Applied Materials & Interfaces·Zhaoyin WangZhihui Dai
Jan 20, 2016·Analytical and Bioanalytical Chemistry·Jingjing DongBaoxin Li
Nov 10, 2015·Chemical Reviews·Yongxi ZhaoChunhai Fan
Oct 18, 2018·Analytical and Bioanalytical Chemistry·Kun WangRong-Huan He

Citations

Feb 29, 2008·Nucleic Acids Research·Jianwei Jeffery LiXiaoliang Sunney Xie
Mar 18, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Kuangwen HsiehH Tom Soh
Dec 7, 2011·Chemical Communications : Chem Comm·Sai BiYangyang Cui

Related Concepts

DNA, Catalytic
Genetic Disorders Screening
Hydrogen Peroxide
DNA Probes
Panhematin
Luminol
Nucleic Acid Amplification Tests
Hemin
Chemistry Techniques, Analytical
Cytokinesis of the Fertilized Ovum

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