Exopolysaccharide and Poly-(beta)-Hydroxybutyrate Coproduction in Two Rhizobium meliloti Strains.

Applied and Environmental Microbiology
P TavernierJ N Barbotin

Abstract

The effects of different nitrogen and carbon sources on cell growth, pH, and exopolysaccharide (EPS) and poly-(beta)-hydroxybutyrate (PHB) production by two strains of Rhizobium meliloti (M5N1 and Su47) are reported. Differences in the behavior of glucose- and fructose-grown cells were shown, in particular with the M5N1 strain. Growth in a glucose-containing medium was accompanied by acidification of the culture medium, which leads to cell death. On fructose, acidification was detected only in the medium with a mineral nitrogen supply. A lag phase in EPS production was observed with cells grown with glucose, probably related to an initial extracellular conversion of the carbohydrate into an acid. No lag phase was observed in EPS production from fructose or in PHB synthesis whatever the carbon source. A decrease in PHB content was noticed for both strains under conditions where acidification of media occurred. The extent of production, emphasized by the use of a coproduction index, indicates that the M5N1 strain is a more promising organism than is the Su47 strain for polymer production. Such a strain, put in rich medium (containing yeast extract) supplemented with fructose, accumulated PHB up to 85% of dry cell weight and excre...Continue Reading

References

Jan 1, 1974·Archives of Microbiology·J J Patel, T Gerson
Oct 1, 1980·Journal of Bacteriology·A GardiolG Martínez-Drets
Jun 1, 1995·Journal of Bacteriology·S EncarnaciónJ Mora
Jun 1, 1994·Microbiological Reviews·M W Breedveld, K J Miller
Aug 1, 1989·Applied and Environmental Microbiology·Graham W O'haraAndrew R Glenn
Jul 1, 1994·Applied and Environmental Microbiology·V I RomanovE Martínez-Romero

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Citations

Mar 27, 2002·Journal of Bacteriology·Sergio EncarnaciónJaime Mora
Aug 3, 2010·FEBS Letters·Arnaud BriqueIsabelle Gosselin
Sep 17, 2008·Applied Biochemistry and Biotechnology·Faouzi Ben RebahLassaad Belbahri
Sep 8, 2010·Applied Biochemistry and Biotechnology·Paulo Ivan FernandesGustavo Ribeiro Xavier
Sep 13, 2013·BioMed Research International·Gulab SinghNeeraj K Aggarwal
Aug 4, 1997·FEBS Letters·J C PortaisJ N Barbotin
Sep 22, 1999·European Journal of Biochemistry·J C PortaisJ N Barbotin
Nov 22, 2001·Journal of Bacteriology·S L López-GarcíaA R Lodeiro
Nov 5, 2002·FEMS Microbiology Reviews·Jean-Charles Portais, Anne-Marie Delort
Jul 18, 2008·FEMS Microbiology Ecology·William C RatcliffRobert Ford Denison
Dec 15, 2020·The Science of the Total Environment·Ayesha Algade AmaduSufeng Wang
Feb 20, 2021·Archives of Microbiology·Berna GencAhmet Adiguzel

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