Exopolysaccharide Features Influence Growth Success in Biocrust-forming Cyanobacteria, Moving From Liquid Culture to Sand Microcosms

Frontiers in Microbiology
Sonia ChamizoRoberto De Philippis

Abstract

Land degradation in drylands is a drawback of the combined action of climate change and human activities. New techniques have been developed to induce artificial biocrusts formation as a tool for restoration of degraded drylands, and among them soils inoculation with cyanobacteria adapted to environmental stress. Improvement of soil properties by cyanobacteria inoculation is largely related to their ability to synthesize exopolysaccharides (EPS). However, cyanobacterial EPS features [amount, molecular weight (MW), composition] can change from one species to another or when grown in different conditions. We investigated the differences in growth and polysaccharidic matrix features among three common biocrust-forming cyanobacteria (Nostoc commune, Scytonema javanicum, and Phormidium ambiguum), when grown in liquid media and on sandy soil microcosms under optimal nutrient and water, in controlled laboratory conditions. We extracted and analyzed the released EPS (RPS) and sheath for the liquid cultures, and the more soluble or loosely-bound (LB) and the more condensed or tightly-bound (TB) soil EPS fractions for the sandy soil microcosms. In liquid culture, P. ambiguum showed the greatest growth and EPS release. In contrast, on the...Continue Reading

References

Mar 8, 2002·Research in Microbiology·Daniela Billi, Malcolm Potts
Apr 17, 2003·Journal of Biotechnology·Ana Otero, Massimo Vincenzini
May 17, 2007·Environmental Microbiology·Erich D Fleming, Richard W Castenholz
May 12, 2012·Journal of Biotechnology·Maria A SinetovaLadislav Nedbal
May 6, 2016·Frontiers in Microbiology·Jay Shankar SinghDevendra P Singh
Jun 20, 2018·The Science of the Total Environment·M Muñoz-RojasY Cantón
Aug 8, 2018·Frontiers in Microbiology·Ohana Y A CostaEiko E Kuramae

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Methods Mentioned

BETA
size exclusion chromatography
Ion Exchange Cromatography
IEC

Software Mentioned

Past
PamWin
EPS
LB
Primer
PERMANOVA

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