Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression

Frontiers in Physiology
Qing Meng, Bing Qiu

Abstract

Rheumatoid arthritis (RA), a chronic systemic inflammatory disease, is a primary cause of disability worldwide. The involvement of fibroblast-like synoviocytes (FLSs) in the regulation of the pathogenesis of RA has been highlighted. Mesenchymal stem cells (MSCs) are important candidates for cell-based treatment in many inflammatory autoimmune diseases. Herein, we identify whether MSC-derived exosomes loaded with microRNA-320a (miR-320a) regulate RA-FLSs. Synovial tissues from 22 patients with RA and 9 patients with osteoarthritis were collected. RA-FLSs were obtained from patients with RA, and their functions were evaluated by determining levels of interleukin-1β (IL-1β), IL-6, and IL-8 and by transwell migration and invasion assays. Dual luciferase reporter gene assays were employed to identify interaction between miR-320a and CXC chemokine ligand 9 (CXCL9). A co-culture system of MSC-derived exosomes and RA-FLSs were performed. The collagen-induced arthritis (CIA) mouse models with arthritis and bone damage were developed. Our results revealed the existence of reciprocal expression of miR-320a and CXCL9 in the synovial tissues obtained from patients with RA. CXCL9 knockdown or miR-320a upregulation suppressed the activation, ...Continue Reading

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Citations

Nov 8, 2020·International Journal of Rheumatic Diseases·Alireza HejratiParisa Zafari
Jan 28, 2021·International Journal of Molecular Sciences·Ju Hun SuhJung Min Lee
May 20, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Chenggui MiaoJinling Huang
Jul 14, 2021·Journal of Translational Medicine·Soudeh MoghadasiMostafa Jarahian
Aug 24, 2021·Ecotoxicology and Environmental Safety·Lixia LiangGuanghai Wang
Oct 1, 2021·Nature Reviews. Rheumatology·Shabana A AliMohit Kapoor

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Datasets Mentioned

BETA
GSE55457

Methods Mentioned

BETA
fluorescence-activated cell sorting
protein assay
electrophoresis
transfection
enzyme-linked immunosorbent assay
ELISA
Assay
flow cytometry
confocal microscopy

Software Mentioned

TargetScan
SPSS
FlowJo

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