Exosomal MicroRNAs Derived from Human Amniotic Epithelial Cells Accelerate Wound Healing by Promoting the Proliferation and Migration of Fibroblasts

Stem Cells International
Bin ZhaoDahai Hu

Abstract

Previous work in our laboratory demonstrated that exosomes derived from human amniotic epithelial cells (hAECs) accelerated wound healing by promoting the proliferation and migration of fibroblasts. It is reported that exosomes, which are carriers of the microRNAs (miRNAs) and proteins, play an important role in the regulation of cell-to-cell communication. However, it is still unclear precisely which molecule or which group of molecules carried within hAEC-derived exosomes (hAEC-Exos) mediated wound healing. Here, we explored purified hAEC-Exos together with either proteinase K (PROse) or RNase A on the effect of fibroblasts and cutaneous wound healing. Our experiments demonstrated that hAEC-Exos were positive for exosomal markers CD9, CD63, and CD81. Also, we found that hAEC-Exos could be internalized by fibroblasts and then stimulated cell migration and proliferation. However, the promotive effect of hAEC-Exos was abolished by pretreating hAEC-Exos with RNase A, not PROse. Importantly, in vivo wound healing assay showed that local injection of hAEC-Exos or PROse pretreated hAEC-Exos at skin wounds significantly accelerated wound healing. Our findings revealed an important role of exosomal miRNAs in wound healing.

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Citations

Apr 3, 2019·International Journal of Molecular Sciences·Giulia GaggiBarbara Ghinassi
Oct 23, 2020·International Journal of Molecular Sciences·Chen QiuJinying Li
Mar 7, 2021·International Journal of Molecular Sciences·Yong MaoJoachim Kohn
Aug 31, 2021·The Journal of Investigative Dermatology·Anesh PrasaiAmina El Ayadi
Nov 23, 2021·Advances in Wound Care·Bibi S SubhanPiul S Rabbani

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Methods Mentioned

BETA
protein assay
electrophoresis
fluorescence microscopy

Software Mentioned

RTCA
ZetaView®
Image Pro Plus
SPSS

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