Exosomal miR-21-5p derived from gastric cancer promotes peritoneal metastasis via mesothelial-to-mesenchymal transition.

Cell Death & Disease
Qiang LiZekuan Xu

Abstract

Peritoneal metastasis is a primary metastatic route for gastric cancers, and the mechanisms underlying this process are still unclear. Peritoneal mesothelial cells (PMCs) undergo mesothelial-to-mesenchymal transition (MMT) to provide a favorable environment for metastatic cancer cells. In this study, we investigated how the exosomal miR-21-5p induces MMT and promotes peritoneal metastasis. Gastric cancer (GC)-derived exosomes were identified by transmission electron microscopy and western blot analysis, then the uptake of exosomes was confirmed by PKH-67 staining. The expression of miR-21-5p and SMAD7 were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot, and the interactions between miR-21-5p and its target genes SMAD7 were confirmed by Luciferase reporter assays. The MMT of PMCs was determined by invasion assays, adhesion assays, immunofluorescent assay, and western blot. Meanwhile, mouse model of tumor peritoneal dissemination model was performed to investigate the role of exosomal miR-21-5p in peritoneal metastasis in vivo. We found that PMCs could internalize GC-derived exosomal miR-21-5p and led to increased levels of miR-21-5p in PMCs. Through various types of in vitro and in vivo a...Continue Reading

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Citations

Feb 6, 2020·Experimental and Therapeutic Medicine·Xiaohong LuShiyun Tan
Aug 12, 2020·Cancer Medicine·Jing Wu, Zuojun Shen
Mar 17, 2019·Molecular Cancer·Min FuXu Zhang
Nov 18, 2019·Critical Reviews in Oncology/hematology·Mara FernandesRui Medeiros
Feb 3, 2021·Journal of Ovarian Research·Shujun CaoXihong Liao
Nov 19, 2020·International Journal of Molecular Sciences·Leona ChitoiuMarieta Costache
Feb 12, 2020·Cancer Letters·Yongwei XiaoTianyu Zhong
Mar 21, 2021·Journal of Experimental & Clinical Cancer Research : CR·Wenkang LuanJinlong Wang
Mar 24, 2021·The International Journal of Biological Markers·Yigang JiaoJingzhe Li
Mar 27, 2021·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Lei DuXiaowei Shen
Apr 3, 2021·Seminars in Cancer Biology·Akira Yokoi, Takahiro Ochiya
Apr 10, 2021·Head & Neck·Gilberto Mendes Menderico JuniorLeandro Luongo de Matos
Jun 11, 2021·Frontiers in Oncology·Jing GaoLian Liu
Jul 27, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Hanyu ZhangMingxing Li

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Datasets Mentioned

BETA
SGC7901
GES-1

Methods Mentioned

BETA
confocal microscopy
dissemination
lavage
PCR
electrophoresis
transfect
transfections

Software Mentioned

Social Sciences ( SPSS )
GraphPad Prism
TargetScan
Image J

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