Exosomal miR-9 Released from HIV Tat Stimulated Astrocytes Mediates Microglial Migration

Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology
Lu YangShilpa Buch

Abstract

Chronic neuroinflammation still remains a common underlying feature of HIV-infected patients on combined anti-retroviral therapy (cART). Previous studies have reported that despite near complete suppression of virus replication by cART, cytotoxic viral proteins such as HIV trans-activating regulatory protein (Tat) continue to persist in tissues such as the brain and the lymph nodes, thereby contributing, in part, to chronic glial activation observed in HIV-associated neurological disorders (HAND). Understanding how the glial cells cross talk to mediate neuropathology is thus of paramount importance. MicroRNAs (miR) also known as regulators of gene expression, have emerged as key paracrine signaling mediators that regulate disease pathogenesis and cellular crosstalk, through their transfer via the extracellular vesicles (EV). In the current study we have identified a novel function of miR-9, that of mediating microglial migration. We demonstrate that miR-9 released from Tat-stimulated astrocytes can be taken up by microglia resulting in their migratory phenotype. Exposure of human astrocytoma (A172) cells to HIV Tat resulted in induction and release of miR-9 in the EVs, which, was taken up by microglia, leading in turn, increase...Continue Reading

References

Apr 6, 2002·Journal of Neurovirology·Ned SacktorLeon Epstein
Apr 23, 2003·Journal of Neurovirology·Justin C McArthurNed Sacktor
May 29, 2003·Journal of Neuropathology and Experimental Neurology·Françoise GrayFrancesco Scaravilli
Apr 23, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ke NingQi Wan
Mar 15, 2006·The Journal of Cell Biology·Amy M BoothStephen J Gould
Jan 2, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ashok ChauhanAvindra Nath
Sep 17, 2008·Oncogene·R Endersby, S J Baker
May 23, 2009·Nature Reviews. Immunology·Serge Rivest
Feb 26, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Slava RomFrancesca Peruzzi
Jan 15, 2011·Journal of Neurovirology·Shiping ZouKurt F Hauser
Mar 4, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Crystal Bethel-BrownShilpa Buch
Mar 25, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Sirosh M BokhariShilpa Buch
Jun 20, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Irena KadiuHoward E Gendelman
May 11, 2013·The Journal of Biological Chemistry·Aarthi NarayananFatah Kashanchi
Jun 12, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amrita Datta ChaudhuriHoward S Fox
Jul 16, 2014·Nature Communications·Honghong YaoShilpa Buch
Aug 20, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D M PegtelS Amor
Feb 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Guohua WangJun Chen
Apr 2, 2015·Experimental Neurology·Joshua E BurdaMichael V Sofroniew
Mar 25, 2016·Current HIV Research·Yu CaiShilpa Buch
Apr 12, 2016·Frontiers in Neuroscience·Manuela Basso, Valentina Bonetto
May 14, 2016·Journal of Neurovirology·Pejman Rahimian, Johnny J He
May 14, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Kai ZhouQing-Wu Yang
Jun 24, 2016·Autophagy·Palsamy PeriyasamyShilpa Buch

❮ Previous
Next ❯

Citations

Jun 23, 2018·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Mithilesh Kumar JhaKyoungho Suk
May 29, 2020·Glia·Dora Brites
Jul 2, 2020·Viruses·Naseer A KutchyShilpa Buch
Nov 5, 2019·Frontiers in Immunology·Aniella MurphyJoan W Berman
Jan 31, 2020·Journal of Extracellular Vesicles·Paula Soledad PérezMatías Ostrowski
Jul 22, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Ke LiaoGuoku Hu
Aug 29, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Susmita SilShilpa Buch
Jun 21, 2019·Cells·Fabio Seiti Yamada YoshikawaLuanda Mara da Silva Oliveira
Aug 12, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Guoku HuShilpa Buch
Nov 17, 2019·Cancers·Michalina ZajdelJan Konrad Siwicki
Apr 28, 2020·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Adeyemi A Olanrewaju, Ramin M Hakami
Nov 26, 2020·International Journal of Molecular Sciences·Morris Losurdo, Mariagrazia Grilli
Apr 15, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Raghavendra UpadhyaAshok K Shetty
Feb 25, 2021·Neuroscience and Biobehavioral Reviews·Shu ZhaoJialin C Zheng
Mar 18, 2021·Ageing Research Reviews·Tongmei ZhangYouming Lu
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Supriya D MahajanJessica L Reynolds
Jul 30, 2021·Current Opinion in HIV and AIDS·Jing ChenWeihua Li

❮ Previous
Next ❯

Methods Mentioned

BETA
Protein Assay
transfection
Assay
PCR
transmission electron microscopy

Software Mentioned

AxioVs 40
Image J

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Ernest T ChiveroShilpa Buch
The Journal of Immunology : Official Journal of the American Association of Immunologists
Crystal Bethel-BrownShilpa Buch
© 2022 Meta ULC. All rights reserved