Exosomal miRNA-215-5p Derived from Adipose-Derived Stem Cells Attenuates Epithelial-Mesenchymal Transition of Podocytes by Inhibiting ZEB2

BioMed Research International
Juan JinQiang He

Abstract

Podocyte migration is actively involved in the process of podocyte loss and proteinuria production, which is closely associated with the development of diabetic nephropathy (DN). Exosomes from adipose-derived stem cells (ADSCs-Exos) effectively inhibit podocyte apoptosis in the treatment of DN. However, how ADSCs-Exos affect the migration of podocytes is obscure. This study is aimed at exploring the regulatory role of ADSCs-Exos on cell migration and the underlying mechanism. ADSCs-Exo was authenticated by transmission electron microscopy (TEM), western blotting, and flow cytometry. Cell viability and migration ability of podocytes were measured by CCK8 and Transwell assays, respectively. Relative expressions of miRNAs and mRNAs were determined by qRT-PCR. The transmitting between PKH26-labeled exosome and podocytes was evaluated by IF assay. Dual luciferase reporter assay was employed to detect the relationship between miR-215-5p and ZEB2. The exposure to serum from DN patient (hDN-serum) significantly inhibited cell viability of podocytes, but ADSCs-Exo addition notably blunts cytotoxicity induced by the transient stimulus of hDN-serum. Besides, ADSCs-Exo administration powerfully impeded high glucose- (HG-) induced migration...Continue Reading

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Citations

Dec 22, 2020·Frontiers in Pharmacology·Linshen Xie, Ye Zeng
Apr 2, 2021·Stem Cell Research & Therapy·Yuling Huang, Lina Yang
May 18, 2021·Frontiers in Endocrinology·Jiachao XiongHua Jiang
Jun 25, 2021·Frontiers in Endocrinology·Yi WangLing-Qing Yuan
Jul 5, 2021·Metabolism: Clinical and Experimental·Jingfang ChenZhangsuo Liu
Aug 6, 2021·Stem Cell Research & Therapy·Yuling Huang, Lina Yang
Oct 15, 2021·Reviews in Endocrine & Metabolic Disorders·Setareh SoltaniReza Yarani
Jul 21, 2021·Journal of Cardiovascular Pharmacology·Xiaojv XiongJijun Liu

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Methods Mentioned

BETA
flow cytometry
transfection
PCR
electron microscopy

Software Mentioned

ImageJ
TargetScan
GraphPad Prism

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