Exosomal proteins as potential diagnostic markers in advanced non-small cell lung carcinoma

Journal of Extracellular Vesicles
Kristine R JakobsenMalene Møller Jørgensen

Abstract

Lung cancer is one of the leading causes of cancer-related death. At the time of diagnosis, more than half of the patients will have disseminated disease and, yet, diagnosing can be challenging. New methods are desired to improve the diagnostic work-up. Exosomes are cell-derived vesicles displaying various proteins on their membrane surfaces. In addition, they are readily available in blood samples where they constitute potential biomarkers of human diseases, such as cancer. Here, we examine the potential of distinguishing non-small cell lung carcinoma (NSCLC) patients from control subjects based on the differential display of exosomal protein markers. Plasma was isolated from 109 NSCLC patients with advanced stage (IIIa-IV) disease and 110 matched control subjects initially suspected of having cancer, but diagnosed to be cancer free. The Extracellular Vesicle Array (EV Array) was used to phenotype exosomes directly from the plasma samples. The array contained 37 antibodies targeting lung cancer-related proteins and was used to capture exosomes, which were visualised with a cocktail of biotin-conjugated CD9, CD63 and CD81 antibodies. The EV Array analysis was capable of detecting and phenotyping exosomes in all samples from onl...Continue Reading

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Methods Mentioned

BETA
biopsies

Software Mentioned

GraphPad
Excel
GraphPad Prism
ROCCET
Genesis

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