Exosomal small RNA sequencing uncovers the microRNA dose markers for power frequency electromagnetic field exposure

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Hualiang LiZhenhua Ding

Abstract

The potential health risks caused by power frequency electromagnetic field (PFEMF) have led to increase public health concerns. However, the diagnosis and prognosis remain challenging in determination of exact dose of PFEMF exposure. Mice were exposed to different magnetic doses of PFEMF for the following isolation of serum exosomes, microRNAs (miRNAs) extraction and small RNA sequencing. After small RNA sequencing, bioinformatic analysis, quantitative real-time PCR (qRT-PCR) validation and serum exosomal miRNA biomarkers were determined. Significantly changed serum exosomal miRNA as biomarkers of 0.1, 0.5, 2.5 mT and common PFEMF exposure were confirmed. Gene ontology (GO) and Kyoto encyclopaedia of genes and genomes (KEGG) pathway analysis of the downstream target genes of the above-identified exosomal miRNA markers indicated that, exosomal miRNA markers were predicted to be involved in critical pathophysiological processes of neural system and cancer- or other disease-related signalling pathways. Aberrantly-expressed serum exosomal miRNAs, including miR-128-3p for 0.1 mT, miR-133a-3p for 0.5 mT, miR-142a-5p for 2.5 mT, miR-218-5p and miR-199a-3p for common PFEMF exposure, suggested a series of informative markers for not onl...Continue Reading

References

Mar 1, 1979·American Journal of Epidemiology·N Wertheimer, E Leeper
Dec 1, 1972·Clinical Biochemistry·R C Kamm, A G Smith
Dec 1, 1995·Journal of Occupational and Environmental Medicine·L I KheifetsZ W Zhang
Aug 17, 2000·British Journal of Cancer·A AhlbomP K Verkasalo
Sep 11, 2003·American Journal of Epidemiology·Niclas HåkanssonBirgitta Floderus
Dec 31, 2003·Cell·Benjamin P LewisChristopher B Burge
Oct 13, 2006·Environmental Health Perspectives·Leeka KheifetsRiti Shimkhada
Jul 3, 2008·Environmental Health : a Global Access Science Source·Martin RöösliChristoph Minder
Sep 30, 2010·British Journal of Cancer·L KheifetsV Wunsch Filho
Feb 10, 2011·British Journal of Cancer·H YoshinoM Nakagawa
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Masayuki KanoHisahiro Matsubara
Dec 14, 2011·The Science of the Total Environment·Stephen J Genuis, Christopher T Lipp
Feb 11, 2012·Nature Reviews. Drug Discovery·Gary J Kelloff, Caroline C Sigman
Apr 16, 2013·Nature Reviews. Drug Discovery·Samir EL AndaloussiMatthew J A Wood
Sep 10, 2013·EMBO Molecular Medicine·Pierre LauBart De Strooper
Dec 7, 2013·Science·Chan Lek TanAnne Schaefer
Oct 7, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A C Lepp, R L Carlone
Nov 8, 2014·Nucleic Acids Research·Nathan Wong, Xiaowei Wang
Nov 28, 2014·Molecular Cancer·Ramesh SinghYin-Yuan Mo
May 15, 2015·Science Translational Medicine·Sanket S AcharyaDipanjan Chowdhury
May 20, 2015·Nature Cell Biology·Bruno Costa-SilvaDavid Lyden
Jul 28, 2015·Nature Communications·Karen P ThiebesSoo-Kyung Lee
Jan 20, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zichao Zhou, Zhijian You
Mar 18, 2017·Journal of Neuroinflammation·Farideh TalebiFarshid Noorbakhsh
Jun 29, 2017·Biochemical and Biophysical Research Communications·Hao-Ran Lv

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.