Exosome-Mediated Small RNA Delivery: A Novel Therapeutic Approach for Inflammatory Lung Responses

Molecular Therapy : the Journal of the American Society of Gene Therapy
Duo ZhangYang Jin

Abstract

Exosomes (EXOs) are a type of extracellular nanovesicles released from living cells. Accumulating evidence suggests that EXOs are involved in the pathogenesis of human diseases, including lung conditions. In recent years, the potential of EXO-mediated drug delivery has gained increasing interest. In this report, we investigated whether inhaled EXOs serve as an efficient and practical delivery vehicle to activate or inhibit alveolar macrophages (AMs), subsequently modulating pulmonary immune responses. We first identified the recipient cells of the inhaled EXOs, which were labeled with PKH26. We found that only lung macrophages efficiently take up intratracheally instilled EXOs in vivo. Using modified calcium chloride-mediated transformation, we manipulated small RNA molecules in serum-derived EXOs, including siRNAs, microRNA (miRNA) mimics, and miRNA inhibitors. Via intratracheal instillation, we successfully delivered siRNA and miRNA mimics or inhibitors into lung macrophages using the serum-derived EXOs as vehicles. Furthermore, EXO siRNA or miRNA molecules are functional in modulating LPS-induced lung inflammation in vivo. Beneficially, serum-derived EXOs themselves do not trigger lung immune responses, adding more favorable...Continue Reading

Citations

Sep 12, 2019·Scientific Reports·Sirisha ThippabhotlaMei He
Sep 27, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Mengdie LiYijie Shi
Jul 11, 2019·Stem Cells Translational Medicine·Golnar ShojaatiJames L Funderburgh
Jun 27, 2019·The Journal of Experimental Medicine·Heedoo LeeYang Jin
Oct 1, 2019·Current Gene Therapy·Ling-Yan ZhouTing Xu
Nov 7, 2019·Drug Development Research·Kamal DuaDinesh Kumar Chellappan
Sep 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Batla Al-SowayanAlaa Alshareeda
Sep 19, 2020·Expert Opinion on Biological Therapy·Manica NegahdaripourNavid Nezafat
Jun 17, 2020·Experimental & Molecular Medicine·Joshua Holtzman, Heedoo Lee
Feb 20, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Heedoo LeeYang Jin
Jun 17, 2020·Expert Opinion on Drug Delivery·Qirong TongGuangxi Zhai
Oct 10, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Victor ChatterjeeSarah Y Yuan
Jan 14, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Heedoo LeeYang Jin
Dec 12, 2018·Journal of Controlled Release : Official Journal of the Controlled Release Society·Heedoo LeeYang Jin
Feb 13, 2021·Small·Fatemeh OroojalianMohammad-Ali Shahbazi
Dec 29, 2020·Journal of Cellular Physiology·Yu-Run LiuZhi-Chong Wang
Jan 13, 2021·International Journal of Molecular Sciences·Laura PastorDavid Sanz-Rubio
Dec 5, 2020·Viruses·Hanna HenzingerMartin Pichler
Dec 5, 2020·Pharmaceutics·Natalia L KlyachkoElena V Batrakova
Nov 13, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ling DingDavid Oupický
Mar 9, 2021·Advanced Nanobiomed Research·Halle LutzKe Cheng
Jun 28, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuzhong ZhengMojtaba Falahati
Mar 23, 2021·Advanced Drug Delivery Reviews·Michaela Schulz-Siegmund, Achim Aigner
Mar 21, 2021·Immunology·Xiaohuan XiaJialin C Zheng
Jul 27, 2021·Advanced Science·Cheng YuJingzhi Ma
Jul 27, 2021·Frontiers in Cell and Developmental Biology·Soudeh Ghafouri-FardAbbas Basiri
Jul 20, 2021·Chemical Reviews·Yuebao ZhangYizhou Dong
Aug 10, 2021·Frontiers in Molecular Biosciences·Xiaoxiao ShanYing Xie
Aug 18, 2021·Acta Pharmaceutica Sinica. B·Makhloufi ZoulikhaWei He
Sep 17, 2021·International Journal of Pharmaceutics·Shilpa Lekhraj Peswani SajnaniDriton Vllasaliu

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