Exosome-mediated transfer of miR-222 is sufficient to increase tumor malignancy in melanoma

Journal of Translational Medicine
F FelicettiA Carè

Abstract

Growing evidence is showing that metastatic cell populations are able to transfer their characteristics to less malignant cells. Exosomes (EXOs) are membrane vesicles of endocytic origin able to convey their cargo of mRNAs, microRNAs (miRs), proteins and lipids from donors to proximal as well as distant acceptor cells. Our previous results indicated that miR-221&222 are key factors for melanoma development and dissemination. The aim of this study was to verify whether the tumorigenic properties associated with miR-222 overexpression can be also propagated by miR-222-containing EXOs. EXOs were isolated by UltraCentrifugation or Exoquick-TC(®) methods. Preparations of melanoma-derived vesicles were characterized by using the Nanosight™ technology and the expression of exosome markers analyzed by western blot. The expression levels of endogenous and exosomal miRNAs were examined by real time PCR. Confocal microscopy was used to evaluate transfer and uptake of microvesicles from donor to recipient cells. The functional significance of exosomal miR-222 was estimated by analyzing the vessel-like process formation, as well as cell cycle rates, invasive and chemotactic capabilities. Besides microvesicle marker characterization, we evid...Continue Reading

References

Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nadia FelliCesare Peschle
Feb 24, 2006·The Journal of Biological Chemistry·Jørgen WescheSjur Olsnes
Oct 19, 2007·Cancer Research·Richard MendelsohnJames W Dennis
Dec 18, 2007·Seminars in Oncology·Ganapati H Mahabeleshwar, Tatiana V Byzova
Feb 28, 2009·Clinical Chemistry·Claudine L Bartels, Gregory J Tsongalis
May 15, 2009·The FEBS Journal·Kazuya TerasawaGozoh Tsujimoto
May 29, 2009·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Jun 13, 2009·International Journal of Cancer. Journal International Du Cancer·Federica FelicettiAlessandra Carè
Dec 8, 2009·Nature Cell Biology·Matias OstrowskiClotilde Thery
Feb 25, 2010·Nature Reviews. Neuroscience·Reha S ErzurumluFilippo M Rijli
Jun 8, 2011·Nature Reviews. Clinical Oncology·Maria Angelica CortezGeorge A Calin
Nov 16, 2011·Current Molecular Medicine·M GarofaloG Condorelli
Feb 18, 2012·Nature·Amaia Lujambio, Scott W Lowe
Jun 5, 2012·Genes & Development·Hensin TsaoDavid E Fisher
Feb 13, 2013·International Journal of Cancer. Journal International Du Cancer·M Cristina ErricoAlessandra Carè
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Jul 11, 2013·International Journal of Molecular Sciences·Bethany N Hannafon, Wei-Qun Ding
Oct 15, 2013·International Journal of Cancer. Journal International Du Cancer·Julien CalderaroYves Allory
Dec 21, 2013·Nature Communications·Carolina Villarroya-BeltriFrancisco Sánchez-Madrid
Jan 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ana L TeixeiraRui Medeiros
Aug 27, 2014·Cellular and Molecular Life Sciences : CMLS·Sarmad N Saleem, Asim B Abdel-Mageed
Aug 28, 2014·Cell Reports·Mario Leonardo SquadritoMichele De Palma
Oct 1, 2014·Journal of Translational Medicine·Chih-Hao ChangAlice Yu
Oct 30, 2014·International Journal of Cancer. Journal International Du Cancer·Bastian SeubertAchim Krüger
Nov 28, 2014·Molecular Cancer·Ramesh SinghYin-Yuan Mo
Jan 19, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sanaa EissaNada O E Essawy
Mar 11, 2015·The Journal of Investigative Dermatology·Erika M YazawaCharles J Dimitroff
Mar 31, 2015·The Journal of Surgical Research·James C LeeStan Sidhu
Sep 1, 2015·Frontiers in Oncology·Giuseppe PalmieriAntonio Cossu

❮ Previous
Next ❯

Citations

Jun 21, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yao WeiLimin Li
Sep 2, 2016·Cellular and Molecular Life Sciences : CMLS·Yutaka NaitoTakahiro Ochiya
Mar 10, 2017·Cellular and Molecular Gastroenterology and Hepatology·Luai R ZarourSkye C Mayo
Jun 27, 2017·The Journal of Investigative Dermatology·Jeffrey D McBrideEvangelos V Badiavas
Mar 23, 2017·Journal of Extracellular Vesicles·Bogdan MateescuEsther N M Nolte-'t Hoen
Sep 25, 2017·Genes·Carlo Maria Di LiegroItalia Di Liegro
Dec 12, 2017·Journal of Cellular Physiology·Casey L RossRina Anvekar
Jan 28, 2018·Targeted Oncology·Marzia PucciSimona Taverna
Mar 6, 2018·Journal of Zhejiang University. Science. B·Zhen-Yu YaoLing Gao
Jun 30, 2018·Journal of Cellular and Molecular Medicine·Li-Li SunXiao-Qiang Li
Aug 6, 2016·PloS One·Niels H H HeegaardJan Fahrenkrug
Nov 10, 2018·International Journal of Cancer. Journal International Du Cancer·Haiyang ZhangYi Ba
Jan 19, 2018·Molecular Cancer·Su Yin LimHelen Rizos
Sep 20, 2018·American Journal of Respiratory Cell and Molecular Biology·Richa GuptaMehmet Kesimer
Feb 5, 2019·Cancer Metastasis Reviews·Mariantonia LogozziStefano Fais
Feb 6, 2020·International Journal of Molecular Sciences·Francesca Varrone, Emilia Caputo
Apr 28, 2017·Biomolecular Concepts·Virginie Armand-Labit, Anne Pradines
Jan 25, 2017·Oncotarget·Luigi FattoreGennaro Ciliberto
Aug 16, 2016·Oncotarget·Yingkuan ShaoShu Zheng
Jul 11, 2020·Cancers·Maria BellenghiGianfranco Mattia
Jul 30, 2020·International Journal of Molecular Sciences·Marta GerasymchukIgor Kovalchuk
Oct 4, 2019·Journal of Extracellular Vesicles·Jia-Tao ZhangWen-Zhao Zhong
Feb 12, 2017·Translational Neurodegeneration·Tingting XiaoLu Shen
Oct 13, 2018·Molecular Cancer·Zhenqiang SunWeitang Yuan
Feb 7, 2019·Journal of Cellular Physiology·Jingjing LiJianda Zhou
Nov 23, 2019·Cells·Emi DikaFederica Scarfì
Jul 28, 2020·International Journal of Molecular Sciences·Aneta ZebrowskaMonika Pietrowska
Aug 14, 2020·Journal of Clinical Medicine·Maria Chiara LionettiCaterina A M La Porta

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Methods Mentioned

BETA
surgical resection
GTPases
genotyping
protein assay
confocal microscopy
antisense oligonucleotides
transfections
flow cytometry

Software Mentioned

AlphaView
GraphPad
Exoquick
Image Quant
NTA
Image J

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