Exosome-Transmitted lncRNA H19 Inhibits the Growth of Pituitary Adenoma

The Journal of Clinical Endocrinology and Metabolism
Yong ZhangZhe Bao Wu

Abstract

Our previous study demonstrated that the expression of long noncoding RNA (lncRNA) H19 was frequently downregulated in human primary pituitary adenomas and negatively correlated with tumor progression. However, the role of exosomal lncRNA H19 in the inhibition of pituitary tumor growth remains unclear. To investigate whether exosomal H19 could be transported across the cell membrane to exert its inhibitory effect on pituitary tumor growth. Empty lentivirus GH3 cells with or without H19 overexpression were used to establish a xenograft model. Isolated exosomes were identified by transmission electron microscopy, nanoparticle tracking, and Western blotting. The expression levels of serum exosomal H19 from 200 healthy subjects and 206 patients with various subtypes of pituitary tumors were detected by ultracentrifugation and quantitative real-time PCR. The growth of distal tumor cells was inhibited by transferring exosomal H19, which could be transported through cell membrane and exert its inhibitory effect. Cabergoline increased H19 expression and played a synergic therapeutic effect with exosomal H19. Exosomal H19 inhibited phosphorylation of the mTORC1 substrate 4E-BP1. Of note, the expression level of exosomal H19 in the patie...Continue Reading

References

May 18, 2006·Endocrine Reviews·Mary P GillamAnnamaria Colao
Oct 7, 2006·Nature Clinical Practice. Endocrinology & Metabolism·Agusta Olafsdottir, Janet Schlechte
Mar 23, 2011·Nature Reviews. Endocrinology·Annamaria Colao, Silvia Savastano
Feb 20, 2013·The Journal of Cell Biology·Clifford V HardingPhilip D Stahl
May 31, 2014·Molecular Cancer Research : MCR·Kenji TakahashiTushar Patel
Jul 30, 2014·Molecular and Cellular Endocrinology·Zhe Bao WuWei Guo Zhao
Sep 23, 2014·Cell Death and Differentiation·C BraicuG A Calin
Oct 4, 2015·Trends in Molecular Medicine·Diana SousaM Helena Vasconcelos
Nov 3, 2015·Nature·Ayuko HoshinoDavid Lyden
Apr 2, 2016·The Journal of Clinical Investigation·Raghu Kalluri
Mar 13, 2017·Journal of Cancer Research and Clinical Oncology·Lei PanXu Zhang
Feb 10, 2018·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xiaojiaoyang LiHuiping Zhou
Feb 25, 2018·Pharmacology & Therapeutics·Maarten P BebelmanS Rubina Baglio
May 26, 2018·Nature Reviews. Clinical Oncology·Rong XuRichard J Simpson
Jan 2, 2019·Nature Cell Biology·Ioanna KeklikoglouMichele De Palma
Jan 6, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hongxia XuWenzhuo Zhuang
Aug 2, 2019·The Journal of Clinical Endocrinology and Metabolism·Yong ZhangZhe Bao Wu

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Citations

Mar 24, 2020·Endocrine Reviews·Vinaya Srirangam Nadhamuni, Márta Korbonits
Jun 6, 2020·Biomedicines·Wilhelm GossingLars Radke
Aug 2, 2019·The Journal of Clinical Endocrinology and Metabolism·Yong ZhangZhe Bao Wu
Nov 23, 2020·Cancer Letters·Yun Wei Shermane LimBoon-Cher Goh
Feb 5, 2021·Molecular and Cellular Endocrinology·Yonghe MaHaiming Cao
Sep 2, 2021·The Journal of Clinical Endocrinology and Metabolism·Cuiqi ZhouShlomo Melmed

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