Exosome-transmitted lncRNA PCGEM1 promotes invasive and metastasis in gastric cancer by maintaining the stability of SNAI1.

Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
H-Y PiaoJ Zhang

Abstract

Clinically, hypoxia is associated with increased distant metastasis and poor survival in gastric cancer (GC). In this study, we set out from the cellular interaction to further explain the molecular mechanism of invasion in GC cells under hypoxic conditions. Gastric cancer cells were cultured under 1% O2 (hypoxia-cultured gastric cancer cells, HGC) and 20% O2 condition (normoxic-cultured gastric cancer cells, NGC). NGC was co-cultured with HGC-medium. Scrape and Transwell were used to evaluate invasion and migration. Exosomes from GC were extracted by ultracentrifugation. Electron microscopy images, western-blot used to analyze the size distributions and the number of exosomes. HGC-medium induced NGC dissociated. Long non-coding RNA (lncRNA) prostate cancer gene expression marker 1 (PCGEM1) was specifically expressed in HGC exosomes. HGC-derived PCGEM1-riched exosomes could promote the invasion and migration of NGC. On the mechanism, PCGEM1 maintained stability and reduced the degradation of SNAI1, which could induce the epithelial-mesenchymal transition of GC. LncRNA PCGEM1 was overexpressed in GC cells. And part of the PCGEM1 can be encapsulated into exosomes. These exosomes promoted invasion and migration of other GC cells. ...Continue Reading

References

Sep 25, 2012·BMC Cancer·Hamish W KingJonathan M Gleadle
Jun 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ting WangGregg L Semenza
Feb 17, 2015·Trends in Cell Biology·Emanuele Cocucci, Jacopo Meldolesi
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Apr 14, 2016·Cancer Cell·Adam M Schmitt, Howard Y Chang
Apr 29, 2016·Science·Erinn B Rankin, Amato J Giaccia
Mar 25, 2017·Experimental Cell Research·Gregg L Semenza
Mar 7, 2018·Journal of Biomedical Science·Ain Zubaidah Ayob, Thamil Selvee Ramasamy
Apr 14, 2018·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Hong XuXianjun Wang
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 29, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·J ZhangY Zhao
Mar 31, 2019·Molecular Cancer·Wanrong MengGuiquan Zhu
Aug 20, 2019·Pathology, Research and Practice·Hong JiangJun Zhang
Aug 31, 2019·BioMed Research International·Jun ZhangYan Zhao

❮ Previous
Next ❯

Citations

May 9, 2021·Archives of Medical Research·Guang-Hui LuGang Sun
Jun 11, 2021·Frontiers in Oncology·Jing GaoLian Liu
Jul 12, 2021·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Hai-Yan PiaoJun Zhang
Jul 27, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Hanyu ZhangMingxing Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
J ZhangYe Zhao
Proceedings of the National Academy of Sciences of the United States of America
V SrikantanShiv Srivastava
Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Shuo ChenYang Zhao
© 2022 Meta ULC. All rights reserved