Exosomes Derived from Bone Mesenchymal Stem Cells Repair Traumatic Spinal Cord Injury by Suppressing the Activation of A1 Neurotoxic Reactive Astrocytes

Journal of Neurotrauma
Wei LiuWeihua Cai

Abstract

Mesenchymal stem cell (MSC) transplantation is now considered as an effective treatment strategy for traumatic spinal cord injury (SCI). However, several key issues remain unresolved, including low survival rates, cell dedifferentiation, and tumor formation. Recent studies have demonstrated that the therapeutic effect of transplanted stem cells is primarily paracrine mediated. Exosomes are an important paracrine factor that can be used as a direct therapeutic agent. However, there are few reports on the application of exosomes derived from bone MSCs (BMSCs-Exos) in treating SCI. In this study, we demonstrated that BMSCs-Exos possessed robust proangiogenic properties, attenuated neuronal cells apoptosis, suppressed glial scar formation, attenuated lesion size, suppressed inflammation, promoted axonal regeneration, and eventually improved functional behavioral recovery effects after traumatic SCI. Briefly, lesion size was decreased by nearly 60%, neuronal apoptosis was attenuated by nearly 70%, glial scar formation was reduced by nearly 75%, average blood vessel density was increased by nearly 60%, and axonal regeneration was increased by almost 80% at day 28 after SCI in the BMSC-Exos group compared to the control group. Using a...Continue Reading

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Citations

Nov 30, 2019·Journal of Molecular Neuroscience : MN·Yuanyuan HeZhongying Chen
Oct 21, 2019·Journal of Neurochemistry·Sergi Roig-PuiggrosAlain Chédotal
Oct 18, 2018·Journal of Clinical Medicine·Bruna CodispotiMarco Tatullo
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Oct 14, 2020·Stem Cells and Development·Zhihua RenRiyi Shi
Feb 10, 2021·Cell Transplantation·Liyi HuangQuan Wei

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Methods Mentioned

BETA
transmission electron microscopy
fluorescence microscopy
dynamic light scattering
dynamic
flow cytometry

Software Mentioned

ParaVision
ImageJ
ZEN lite

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