Exosomes derived from mesenchymal stem cells inhibit neointimal hyperplasia by activating the Erk1/2 signalling pathway in rats.

Stem Cell Research & Therapy
Zhihui LiuTao Jing

Abstract

Restenosis is a serious problem in patients who have undergone percutaneous transluminal angioplasty. Endothelial injury resulting from surgery can lead to endothelial dysfunction and neointimal formation by inducing aberrant proliferation and migration of vascular smooth muscle cells. Exosomes secreted by mesenchymal stem cells have been a hot topic in cardioprotective research. However, to date, exosomes derived from mesenchymal stem cells (MSC-Exo) have rarely been reported in association with restenosis after artery injury. The aim of this study was to investigate whether MSC-Exo inhibit neointimal hyperplasia in a rat model of carotid artery balloon-induced injury and, if so, to explore the underlying mechanisms. Characterization of MSC-Exo immunophenotypes was performed by electron microscopy, nanoparticle tracking analysis and western blot assays. To investigate whether MSC-Exo inhibited neointimal hyperplasia, rats were intravenously injected with normal saline or MSC-Exo after carotid artery balloon-induced injury. Haematoxylin-eosin staining was performed to examine the intimal and media areas. Evans blue dye staining was performed to examine re-endothelialization. Moreover, immunohistochemistry and immunofluorescence...Continue Reading

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Citations

Mar 21, 2021·Medical Oncology·Ameneh JafariMostafa Rezaei-Tavirani
Jun 2, 2021·DNA and Cell Biology·Lin-Zhen XiaZuo Wang
Jun 10, 2021·Journal of Tissue Engineering·Jung-Hwan LeeHae-Won Kim

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Methods Mentioned

BETA
percutaneous transluminal angioplasty
flow cytometry
protein assay
transmission electron microscopy
electrophoresis
dissection

Software Mentioned

Image
Pro Plus

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