Exosomes derived from microRNA-199a-overexpressing mesenchymal stem cells inhibit glioma progression by down-regulating AGAP2

Aging
Lei YuBinghui Qiu

Abstract

Accumulating evidence has implied that microRNAs (miRNAs) are implicated in glioma progression, and genetically engineered mesenchymal stem cells can help to inhibit tumor growth of glioma. Herein we hypothesized that miR-199a could be delivered by mesenchymal stem cells to glioma cells through exosomes and thus prevent the glioma development by down-regulating ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2). The expression pattern of miR-199a and AGAP2 was characterized in glioma tissues and cells using RNA polymerase chain reaction quantification, immunohistochemical staining and Western blot assays. Mesenchymal stem cells transfected with miR-199a mimic or their derived exosomes were co-cultured with U251 cells. The biological behaviors as well as chemosensitivity of U251 cells were assessed to explore the involvement of miR-199a/AGAP2 in glioma. MiR-199a was poorly expressed in glioma tissue and cells while AGAP2 was highly expressed. Mesenchymal stem cells delivered miR-199a to the glioma cells via the exosomes, which resulted in the suppression of the proliferation, invasion and migration of glioma cells. Besides, mesenchymal stem cells over-expressing miR-199a enhanced the chemosensitivity to temozolomi...Continue Reading

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Citations

Sep 23, 2020·International Journal of Molecular Sciences·Narasimha M BeerakaGjumrakch Aliev
Sep 26, 2020·Frontiers in Cell and Developmental Biology·Patricia Kauanna Fonseca DamascenoMilena Botelho Pereira Soares
Apr 21, 2020·Current Pharmaceutical Design·Guifang ZhaoWenliang Li
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Jan 26, 2020·International Journal of Molecular Sciences·Hyeon Su JooJung Min Lee
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Jan 6, 2021·Stem Cell Reviews and Reports·Zafer CetinEmel Sokullu
Dec 29, 2020·Journal of Cellular Physiology·Yu-Run LiuZhi-Chong Wang
Apr 19, 2021·Seminars in Cell & Developmental Biology·Priya MondalSyed Musthapa Meeran

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Datasets Mentioned

BETA
GSE79097

Methods Mentioned

BETA
surgical resection
flow cytometry
transfection
fluorescence microscopy
electron microscopy
electrophoresis
xenograft

Software Mentioned

R
limma
SPSS
Target Scan
language

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