Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII

Oxidative Medicine and Cellular Longevity
Yan WangBei Shi

Abstract

Cardiac stem cells (CSCs) have emerged as one of the most promising stem cells for cardiac protection. Recently, exosomes from bone marrow-derived mesenchymal stem cells (BMSCs) have been found to facilitate cell proliferation and survival by transporting various bioactive molecules, including microRNAs (miRs). In this study, we found that BMSC-derived exosomes (BMSC-exos) significantly decreased apoptosis rates and reactive oxygen species (ROS) production in CSCs after oxidative stress injury. Moreover, a stronger effect was induced by exosomes collected from BMSCs cultured under hypoxic conditions (Hypoxic-exos) than those collected from BMSCs cultured under normal conditions (Nor-exos). We also observed greater miR-214 enrichment in Hypoxic-exos than in Nor-exos. In addition, a miR-214 inhibitor or mimics added to modulate miR-214 levels in BMSC-exos revealed that exosomes from miR-214-depleted BMSCs partially reversed the effects of hypoxia-induced exosomes on oxidative damage in CSCs. These data further confirmed that miR-214 is the main effector molecule in BMSC-exos that protects CSCs from oxidative damage. miR-214 mimic and inhibitor transfection assays verified that CaMKII is a target gene of miR-214 in CSCs, with exos...Continue Reading

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Citations

Jun 25, 2020·International Journal of Molecular Sciences·Montserrat ClimentAlessandra Castaldi
Mar 28, 2019·Frontiers in Neurology·Oh Young Bang, Eun Hee Kim
Aug 27, 2019·Frontiers in Cell and Developmental Biology·Amanda LeitolisMarco A Stimamiglio
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Dec 29, 2020·Frontiers in Pharmacology·Jia LiuJunnan Wang
Mar 29, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Ramiro A Villarreal-LealBruna Corradetti
Apr 20, 2021·Advanced Drug Delivery Reviews·Anika NagelkerkeMolly M Stevens
May 10, 2021·Free Radical Biology & Medicine·Gabriel Palermo RuizMarcelo A Mori
Jun 12, 2021·Stem Cell Reports·Si-Jia SunHung-Fat Tse
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Xun WuChengming Fan
Dec 26, 2021·Journal of Cellular and Molecular Medicine·Chao WenChenxia Hu

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Methods Mentioned

BETA
protein assay
transfection
Infection
fluorescence microscopy
PCR
Assay
MDA
electrophoresis

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