Exosomes derived from miR-375-overexpressing human adipose mesenchymal stem cells promote bone regeneration

Cell Proliferation
Si ChenYongsheng Zhou

Abstract

The present study aimed to investigate whether exosomes derived from miR-375-overexpressing human adipose mesenchymal stem cells (hASCs) could enhance bone regeneration. Exosomes enriched with miR-375 (Exo [miR-375]) were generated from hASCs stably overexpressing miR-375 after lentiviral transfection and identified with transmission electron microscopy, nanosight and western blotting. The construction efficiency of Exo (miR-375) was evaluated with qRT-PCR and incubated with human bone marrow mesenchymal stem cells (hBMSCs) to optimize the effective dosage. Then, the osteogenic capability of Exo (miR-375) was investigated with ALP and ARS assays. Furthermore, dual-luciferase reporter assay and western blotting were conducted to reveal the underlying mechanism of miR-375 in osteogenic regulation. Finally, Exo (miR-375) were embedded with hydrogel and applied to a rat model of calvarial defect, and μ-CT analysis and histological examination were conducted to evaluate the therapeutic effects of Exo (miR-375) in bone regeneration. miR-375 could be enriched in exosomes by overexpressing in the parent cells. Administration of Exo (miR-375) at 50 μg/mL improved the osteogenic differentiation of hBMSCs. With miR-375 absorbed by hBMSCs,...Continue Reading

References

Jul 2, 2003·Cells, Tissues, Organs·Daniel A De UgarteMarc H Hedrick
Jan 18, 2006·Stem Cells·Kevin McIntoshJeffrey M Gimble
Nov 16, 2007·American Journal of Physiology. Endocrinology and Metabolism·Cheryl A Conover
Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Jun 24, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dongmei SunHuang-Ge Zhang
Nov 26, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yukihiro AkaoTomoki Naoe
Mar 23, 2011·Journal of Translational Medicine·Carla A HerbertsHarm P H Hermsen
Mar 23, 2011·Nature Biotechnology·Lydia Alvarez-ErvitiMatthew J A Wood
Jul 6, 2011·Cell Stem Cell·Arnold I Caplan, Diego Correa
Feb 1, 2012·Nature Reviews. Endocrinology·Jane B LianYing Zhang
Feb 9, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Casey A MaguireJohan Skog
Jul 14, 2012·World Journal of Gastroenterology : WJG·Eric Verna
Aug 23, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Arda MizrakOkay Saydam
Oct 4, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shin-ichiro OhnoMasahiko Kuroda
Jun 7, 2014·Annual Review of Biomedical Engineering·Martin L YarmushDamijan Miklavčič
Jul 17, 2014·Nature Reviews. Molecular Cell Biology·Minju Ha, V Narry Kim
Oct 9, 2014·Annual Review of Pharmacology and Toxicology·Bence GyörgyJoshua N Leonard
Oct 9, 2014·Annual Review of Physiology·Stella Kourembanas
Jul 6, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Lara MilaneMansoor M Amiji
Apr 19, 2016·Journal of Pediatric Endocrinology & Metabolism : JPEM·Heike Hoyer-KuhnOliver Semler
Oct 19, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dominique IngatoYoung Jik Kwon
Jan 8, 2017·Journal of Cellular Physiology·Fahimeh ShahabipourAmirhossein Sahebkar
Jun 13, 2017·International Journal of Molecular Sciences·Mauro CataldiDomenico Capone
Jun 28, 2017·Stem Cells Translational Medicine·Bi ChenYang Wang
Jul 26, 2017·Cell Proliferation·Zi-Chen HaoShuo-Gui Xu
Jan 24, 2018·ACS Applied Materials & Interfaces·Wenyue LiYongsheng Zhou
Feb 17, 2018·Trends in Molecular Medicine·Ivano Luigi ColaoIvan Wall
Aug 21, 2018·Frontiers in Endocrinology·Jacob O'BrienChun Peng

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Citations

Jan 26, 2020·International Journal of Molecular Sciences·Hyeon Su JooJung Min Lee
May 27, 2020·Journal of Cellular Physiology·Shirin TavakoliAdel Naimi
Aug 12, 2020·Basic & Clinical Pharmacology & Toxicology·Xiaoqin Wang, Peter Thomsen
May 28, 2020·Nature Reviews. Molecular Cell Biology·Killian O'BrienXandra O Breakefield
Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Hui-Chun YanDa-Wei Liu
Nov 20, 2020·Cell Proliferation·Yuhe JiangYongsheng Zhou
Jan 28, 2021·Methods and Protocols·Ishani Dasgupta, Anushila Chatterjee
Nov 20, 2020·Bone·David E KomatsuMichael Hadjiargyrou
Feb 6, 2021·Stem Cells International·Bei YinLing Ye
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Benjamin GantenbeinDevina Purmessur
Jan 18, 2021·Acta Biomaterialia·Vishnu Priya Murali, Christina A Holmes
Mar 7, 2021·Journal of Clinical Medicine·Byung-Chul LeeKyung-Rok Yu
Dec 11, 2020·Tissue Engineering. Part B, Reviews·Julika HuberNatalina Quarto
Mar 22, 2021·Life Sciences·Yang LiuChaolai Man
Mar 23, 2021·Advanced Drug Delivery Reviews·Michaela Schulz-Siegmund, Achim Aigner
Apr 28, 2021·Stem Cell Reviews and Reports·Maria Luz Alonso-AlonsoYolanda Diebold
Apr 20, 2021·Advanced Drug Delivery Reviews·Anika NagelkerkeMolly M Stevens
May 28, 2021·Journal of Leukocyte Biology·Zhiyu ZhangJianxia Tang
Jun 3, 2021·International Journal of Molecular Sciences·Agnese GugliandoloFrancesca Diomede
Jun 8, 2021·Bone Reports·Vishnu Priya Murali, Christina A Holmes

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Methods Mentioned

BETA
Protein Assay
transmission electron microscopy
reverse transcription PCR
Assay
transfection
fluorescence microscopy
genetic modification

Software Mentioned

NTA
RNA22
Inveon Research Workplace
Nanosight
zetaview

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