Exosomes from C2C12 myoblasts enhance osteogenic differentiation of MC3T3-E1 pre-osteoblasts by delivering miR-27a-3p

Biochemical and Biophysical Research Communications
Qian XuJinxiang Han

Abstract

Many regulators have been identified to participate in the cross-talk between muscle and bone, however, most previous studies focus on secreting proteins. In this study, we demonstrated that exosomes from myoblasts C2C12 can promote pre-osteoblasts MC3T3-E1 differentiation to osteoblasts. We revealed that the effect of C2C12 exosomes depended on its miR-27a-3p component, they can increase miR-27a-3p level in the recipient cells, and decrease its direct target adenomatous polyposis coli (APC) expression, thus activating β-catenin pathway. Furthermore, C2C12 exosomes failed to exert above effects when miR-27a-3p was deprived. These findings indicates exosomal microRNAs can be regarded as a novel type of "myokines" with osteogenesis promoting potential, which would broad our understanding of the muscle-bone interaction under physiological and pathological conditions.

Citations

Jul 1, 2020·Connective Tissue Research·Khairat Bahgat Youssef El Baradie, Mark W Hamrick
Feb 6, 2020·Frontiers in Physiology·Lyndon F CooperMiya Kang
Sep 6, 2019·Disease Markers·Christos Masaoutis, Stamatios Theocharis
Nov 20, 2019·Current Osteoporosis Reports·Weiping Qin, Sarah L Dallas
Sep 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Batla Al-SowayanAlaa Alshareeda
Aug 27, 2019·Frontiers in Physiology·Sophie RomeKarim Bouzakri
Oct 13, 2020·Frontiers in Bioengineering and Biotechnology·Li WangHongyan Guo
Nov 27, 2020·Journal of Cellular Physiology·Qi ShangHui Ren
Feb 12, 2021·Journal of Molecular Neuroscience : MN·Hongyu LuQuantang Pang
Dec 29, 2020·Frontiers in Cell and Developmental Biology·Chen HeChangjun Li
Feb 6, 2021·Stem Cells International·Bei YinLing Ye
Apr 20, 2021·Advanced Drug Delivery Reviews·Anika NagelkerkeMolly M Stevens
May 18, 2021·Intractable & Rare Diseases Research·Hui ZhangJinxiang Han
Jul 3, 2021·International Journal of Molecular Sciences·Marco Ponzetti, Nadia Rucci

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