Exosomes Secreted by Adipose-Derived Mesenchymal Stem Cells Foster Metastasis and Osteosarcoma Proliferation by Increasing COLGALT2 Expression

Frontiers in Cell and Developmental Biology
Yan WangBohua Chen

Abstract

Homosapien collagen beta (1-O) galactosyl transferase 2 (COLGALT2) is an important enzyme during collagen glycosylation, yet its biological functions in cancer are incompletely understood. Our previous study revealed that in the osteosarcoma microenvironment, adipose-derived mesenchymal stem cells (ADSCs) demonstrate cancer-promoting effects, but the exact mechanisms remain unclear. The aim of this study was to investigate the role of COLGALT2 in the osteosarcoma-fostering effects of ADSCs. In this study, we compared COLGALT2 expression between primary and metastatic osteosarcoma tissues and found that metastatic tissues expressed significantly higher COLGALT2 levels. Then, we isolated and identified exosomes secreted by ADSCs. Additionally, we assessed the roles of ADSC exosomes and COLGALT2 in the osteosarcoma-promoting effects of ADSCs. Our results showed that ADSC exosomes could foster the invasion, migration, and proliferation of osteosarcoma cells, together with increasing COLGALT2 expression. COLGALT2 inhibition in MG63 cells suppressed the ADSC exosome-mediated fostering of osteosarcoma cell invasion, migration and proliferation in vitro. Conversely, COLGALT2 overexpression promoted U-2OS cell invasion, migration and pr...Continue Reading

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Citations

Dec 20, 2020·Cancers·Stephanie AnnettTracy Robson
Mar 31, 2021·Clinical and Translational Medicine·Ting GuoCongjian Xu
Apr 5, 2021·Biochemical and Biophysical Research Communications·Dandan WeiShiqing Jiang
Jul 3, 2021·Bioengineering·Banani KunduSubhas C Kundu
Jul 15, 2021·Journal of Bone Oncology·Xingyu ChangJingjing Yang
Nov 18, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yan LiYi-Hao Wang

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Methods Mentioned

BETA
glycosylation
protein assay
Assay
PCR
xenograft
electron microscopy
xenografts

Software Mentioned

Image
Pro Plus
Living Image
Image Lab TM

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