Exotoxin diversity of Staphylococcus aureus isolated from milk of cows with subclinical mastitis in Central Russia

Journal of Dairy Science
K K FursovaF A Brovko

Abstract

Mastitis, a major veterinary problem widespread in many regions, is caused mainly by Staphylococcus spp. However, there is no current reliable information about the role of Staphylococcus aureus and their toxins in the development of mastitis in cows in the territory of the Russian Federation. The aim of this investigation was to determine the profile of exotoxins of S. aureus from cow milk from farms of Central Russia. A total of 60 isolates of S. aureus were obtained from milk samples of cows with the subclinical form of mastitis. The exotoxin genes were identified using 2 types of PCR assays. The diversity of enterotoxin genes was studied by multiplex PCR. The percentage occurrence of enterotoxin genes was as follows: sea, 53.3%; seb, 3.3%; sec, 50%; sed, 4%; see, 46.6%; seg, 70%; sei, 10%; selp, 3.3%; and tsst1, 1.6%. The seh gene was not detected. The genes of pore-forming toxins and phenol-soluble modulins were identified by singleplex PCR and consisted of the following: hlA, 70%; lucS, 46.6%; psmA, 81.6%; psmB, 95%; and hld, 78.3%. The most abundant genes were psm (psmB, 95%), which codes for pore-forming toxins, and seg (70%), which codes for enterotoxins. The production of some enterotoxins in bacterial culture medium ...Continue Reading

References

Aug 25, 2012·Frontiers in Cellular and Infection Microbiology·François VandeneschThomas Henry
Sep 11, 2013·Nature Reviews. Microbiology·Andreas Peschel, Michael Otto
Nov 26, 2013·Nature Immunology·Arby AbtinWolfgang Weninger
May 13, 2015·Infection and Immunity·Sana S DastgheybMichael Otto
Nov 1, 2016·MBio·Hwang-Soo JooMichael Otto
Jun 13, 2017·Nature Reviews. Microbiology·Hilary P BrowneTrevor D Lawley

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