Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction

Stem Cells International
Alejandro CorreaPaulo Roberto Slud Brofman

Abstract

Pharmacological approaches are partially effective in limiting infarct size. Cell therapies using a cell population enriched with endothelial progenitor cells (EPCs) CD133+ have opened new perspectives for the treatment of ischemic areas after infarction. This preclinical study evaluated the effect of intramyocardial transplantation of purified or expanded human umbilical cord blood-derived CD133+ cells on the recovery of rats following acute myocardial infarction (AMI). Histology studies, electrocardiogram, and fluorescence in situ hybridization (FISH) were used to evaluate heart recovery. Purified CD133+ cells, enriched in endothelial progenitor cells, when expanded in vitro acquired an endothelial-like cell phenotype expressing CD31 and von Willebrand factor (vWF). The group of infarcted rats that received expanded CD133+ cells had a more significant recovery of contraction performance and less heart remodeling than the group that received purified CD133+ cells. Either purified or expanded CD133+ cells were able to induce neovascularization in the infarcted myocardium in an equivalent manner. Few human cells were detected in the infarcted myocardium of the rats 28 days after transplantation suggesting that the effects observ...Continue Reading

References

Sep 2, 1993·The New England Journal of Medicine·UNKNOWN GUSTO investigators
Jun 1, 1996·Heart·N B Schiller, E Foster
Jul 21, 2004·Journal of the American College of Cardiology·Onnik AgbulutPhilippe Menasché
Aug 3, 2004·Revista da Associação Médica Brasileira·Antonio Eduardo Pereira PesaroJosé Carlos Nicolau
Feb 5, 2005·Cell Transplantation·Robert J HenningPaul R Sanberg
Dec 18, 2007·Experimental Cell Research·Nana MukaiIkuo Morita
Nov 21, 2009·Circulation Journal : Official Journal of the Japanese Circulation Society·Bernhard SchlechtaAlfred A Kocher
Apr 21, 2010·Experimental Biology and Medicine·Alexandra C SenegagliaPaulo R S Brofman
Jun 10, 2010·Clinical and Experimental Pharmacology & Physiology·Cheng-Heng HuGui-Fu Wu
Jul 5, 2012·Cold Spring Harbor Perspectives in Medicine·Mervin C Yoder
Feb 26, 2015·Revista brasileira de cirurgia cardiovascular : órgão oficial da Sociedade Brasileira de Cirurgia Cardiovascular·Luiz Guilherme Achcar CapriglionePaulo Roberto Brofman
Apr 23, 2015·Stem Cell Research·Lachlan M MoldenhauerClaudine S Bonder

❮ Previous
Next ❯

Methods Mentioned

BETA
percutaneous coronary angioplasty
flow cytometry

Software Mentioned

FlowJo
analySIS getIT

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.