Expanded CTG repeats in myotonin protein kinase suppresses myogenic differentiation

Neuroreport
F UsukiK Suzuki

Abstract

A full-length mutant human myotonin protein kinase (MtPK) cDNA having a 46 expanded CTG-repeat size or a wild type containing 5 CTG repeats was stably transfected into mouse C2C12 cell line in order to explore the effects of the expansion mutation of trinucleotide repeats in the 3' untranslated region on developing myogenic cells. Each established clone expressed a human 70 kDa MtPK protein without proteolytic processing. Differentiation experiments indicated that stable mutant MtPK cDNA-transformants suppressed myogenic differentiation, whereas wild-type transformants exhibited almost normal myogenesis. The disturbance of the expression of neuronal nitric oxide synthase, a mediator of myoblast fusion, suggests that signal transduction system might be involved in the muscle manifestations of mutant MtPK.

Citations

Jun 6, 2007·Journal of Neuroscience Research·Mario Bermúdez de León, Bulmaro Cisneros
Oct 21, 1999·Biochemical and Biophysical Research Communications·N SasagawaS Ishiura
May 1, 2010·Cell Death and Differentiation·E LoroL Vergani

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