Expanded repertoire of AAV vector serotypes mediate unique patterns of transduction in mouse brain.

Molecular Therapy : the Journal of the American Society of Gene Therapy
Cassia N CearleyJohn H Wolfe

Abstract

A wide diversity of adeno-associated virus (AAV) structural proteins uncovered from latent genomes in primate tissue has expanded the number of AAV vector serotypes, which can potentially confer unique cell tropism to the vector. We evaluated 17 of these vectors in the mouse brain using green fluorescent protein (GFP) as a reporter gene. A rapid initial evaluation was performed by neonatal lateral ventricle injections. Vectors made with capsids hu.32, hu.37, pi.2, hu.11, rh.8, hu.48R3, and AAV9 for comparison were selected for further analysis based on their ability to transduce large numbers of cells and result in novel patterns of cell transduction. These vectors were injected into adult brains in four major structures (cortex, striatum, hippocampus, and thalamus), and all were found to transduce neurons. In addition, hu.32, hu.11, pi.2, hu.48R3, and rh.8 resulted in GFP expression in some astrocytes or oligodendrocytes. AAVs rh.8, pi.2, hu.32, and hu.11 also appeared to result in neuronal transport of the vector genome. Vector transport was studied by a single unilateral injection into the hippocampus and vector genome was found in projection sites of the hippocampus. These unique patterns of cell transduction expand the pot...Continue Reading

References

Apr 15, 1979·The Journal of Comparative Neurology·S Laurberg
Jul 10, 1981·The Journal of Comparative Neurology·J M Wyss
Sep 22, 1999·Gene Therapy·N ChirmuleJ Wilson
Sep 29, 1999·Journal of Medical Virology·K ErlesJ R Schlehofer
Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·B L DavidsonJ A Chiorini
Jul 23, 2002·Journal of Virology·Mihail Y MastakovMatthew J During
Aug 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Guang-Ping GaoJames M Wilson
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Guangping GaoJames M Wilson
Aug 15, 1979·The Journal of Comparative Neurology·L W Swanson, W M Cowan
May 28, 2004·Journal of Virology·Guangping GaoJames M Wilson
Apr 27, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Marco A PassiniGregory R Stewart
Jan 18, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Cassia N Cearley, John H Wolfe
Jun 30, 2006·Mental Retardation and Developmental Disabilities Research Reviews·David PleasurePeter Bannerman
Jul 18, 2006·Nature Medicine·Luk H VandenbergheJames M Wilson
Nov 23, 2006·Nature Clinical Practice. Neurology·Nicholas J Maragakis, Jeffrey D Rothstein
Dec 5, 2006·The International Journal of Biochemistry & Cell Biology·Fei He, Yi E Sun
Mar 9, 2007·Human Gene Therapy·Jean-Marc TaymansVeerle Baekelandt

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Citations

Jul 21, 2009·Journal of Neuro-oncology·Casey A MaguireMiguel Sena-Esteves
Oct 22, 2008·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Kathleen A Maguire-Zeiss, Howard J Federoff
Jul 30, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Patricia A LawlorMatthew J During
Sep 24, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hemraj B DodiyaJeffrey H Kordower
Dec 17, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Eleni A MarkakisD Eugene Redmond
Apr 14, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Steven J GrayR Jude Samulski
Jun 2, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·John R Forsayeth, Krystof S Bankiewicz
May 26, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hongwei ZhangGuangping Gao
May 17, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yong Hong ChenBeverly L Davidson
Jun 20, 2013·Molecular Therapy. Nucleic Acids·Casey A MaguireBakhos A Tannous
Dec 23, 2008·Nature Biotechnology·Kevin D FoustBrian K Kaspar
Jun 15, 2012·Human Gene Therapy Methods·Mai K ElMallahDavid D Fuller
Jan 16, 2010·Human Gene Therapy·Marijke W A de BackerRoger A H Adan
Mar 25, 2011·Human Molecular Genetics·William J BowersMiguel Sena-Esteves
Sep 3, 2011·Science·Xiaoke ChenCharles S Zuker
Nov 19, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jose F AbisambraChad A Dickey
Feb 8, 2014·Nature Medicine·Jing L Guo, Virginia M Y Lee
Apr 1, 2011·Therapeutic Delivery·Ahad A RahimSimon N Waddington
Oct 9, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michael J CastleJohn H Wolfe
Apr 29, 2014·Molecular and Cellular Neurosciences·Josef PletichaAndreas S Beutler
May 2, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bin YangGuangping Gao
Aug 28, 2014·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Casey A MaguireXandra O Breakefield
Jan 22, 2013·British Journal of Clinical Pharmacology·Abdelwahed ChtartoLiliane Tenenbaum
Oct 30, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·David A HartmannAndy Y Shih
Nov 19, 2013·Behavioural Brain Research·Christopher Janus, Todd Golde

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