Diagnostic tests for fish allergy are hampered by the large number of under-investigated fish species. Four salmon allergens are well-characterized and registered with the WHO/IUIS while no catfish allergens have been described so far. In 2008, freshwater-cultured catfish production surpassed that of salmon, the globally most-cultured marine species. We aimed to identify, quantify, and compare all IgE-binding proteins in salmon and catfish. Seventy-seven pediatric patients with clinically confirmed fish allergy underwent skin prick tests to salmon and catfish. The allergen repertoire of raw and heated protein extracts was evaluated by immunoblotting using five allergen-specific antibodies and patients' serum followed by mass spectrometric analyses. Raw and heated extracts from catfish displayed a higher frequency of IgE-binding compared to those from salmon (77% vs 70% and 64% vs 53%, respectively). The major fish allergen parvalbumin demonstrated the highest IgE-binding capacity (10%-49%), followed by triosephosphate isomerase (TPI; 19%-34%) in raw and tropomyosin (6%-32%) in heated extracts. Six previously unidentified fish allergens, including TPI, were registered with the WHO/IUIS. Creatine kinase from salmon and catfish wa...Continue Reading
Comparative study of GH-transgenic and non-transgenic amago salmon (Oncorhynchus masou ishikawae) allergenicity and proteomic analysis of amago salmon allergens
Characterization of allergens isolated from the freshwater fish blunt snout bream (Megalobrama amblycephala)
Identification of sole parvalbumin as a major allergen: study of cross-reactivity between parvalbumins in a Spanish fish-allergic population
Identification of enolases and aldolases as important fish allergens in cod, salmon and tuna: component resolved diagnosis using parvalbumin and the new allergens
Skin prick test responses and allergen-specific IgE levels as predictors of peanut, egg, and sesame allergy in infants
Identification of parvalbumin and two new thermolabile major allergens of Thunnus tonggol using a proteomics approach
Molecular and immunological approaches in quantifying the air-borne food allergen tropomyosin in crab processing facilities
Differential IgE binding to isoallergens from Asian seabass (Lates calcarifer) in children and adults
Immunological cross-reactivity between four distant parvalbumins-Impact on allergen detection and diagnostics
Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management
Characterization of Ras k 1 a novel major allergen in Indian mackerel and identification of parvalbumin as the major fish allergen in 33 Asia-Pacific fish species
Occupational Asthma and Urticaria in a Fishmonger Due to Creatine Kinase, a Cross-Reactive Fish Allergen
Rapid and comprehensive discovery of unreported shellfish allergens using large-scale transcriptomic and proteomic resources
Diagnostic accuracy, risk assessment, and cost-effectiveness of component-resolved diagnostics for food allergy: A systematic review
A cross-sectional, population-based study on the prevalence of food allergies among children in two different socio-economic regions of Vietnam
Commercial fish ELISA kits have a limited capacity to detect different fish species and their products
Effects of Extraction Buffer on the Solubility and Immunoreactivity of the Pacific Oyster Allergens.
African American Children Are More Likely to Be Allergic to Shellfish and Finfish: Findings from FORWARD, a Multisite Cohort Study.
Allergy and Asthma
Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.