Expanding the Diet for DIET: Electron Donors Supporting Direct Interspecies Electron Transfer (DIET) in Defined Co-Cultures

Frontiers in Microbiology
Liying WangDerek R Lovley

Abstract

Direct interspecies electron transfer (DIET) has been recognized as an alternative to interspecies H2 transfer as a mechanism for syntrophic growth, but previous studies on DIET with defined co-cultures have only documented DIET with ethanol as the electron donor in the absence of conductive materials. Co-cultures of Geobacter metallireducens and Geobacter sulfurreducens metabolized propanol, butanol, propionate, and butyrate with the reduction of fumarate to succinate. G. metallireducens utilized each of these substrates whereas only electrons available from DIET supported G. sulfurreducens respiration. A co-culture of G. metallireducens and a strain of G. sulfurreducens that could not metabolize acetate oxidized acetate with fumarate as the electron acceptor, demonstrating that acetate can also be syntrophically metabolized via DIET. A co-culture of G. metallireducens and Methanosaeta harundinacea previously shown to syntrophically convert ethanol to methane via DIET metabolized propanol or butanol as the sole electron donor, but not propionate or butyrate. The stoichiometric accumulation of propionate or butyrate in the propanol- or butanol-fed cultures demonstrated that M. harundinaceae could conserve energy to support grow...Continue Reading

References

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Oct 7, 2014·Bioresource Technology·Shanshan ChenDerek R Lovley

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Citations

Apr 21, 2017·International Journal of Molecular Sciences·Ramiro Blasco-GómezSebastià Puig
Jan 15, 2017·Applied and Environmental Microbiology·Anna ProkhorovaJohannes Gescher
Aug 8, 2018·Journal of Bacteriology·Derek R Lovley
Nov 5, 2016·FEMS Microbiology Ecology·Tillmann Lueders
Feb 23, 2020·Frontiers in Bioengineering and Biotechnology·Tianwen ZhengMin Jiang
Dec 12, 2020·Frontiers in Microbiology·Krisztián LacziKatalin Perei
Dec 10, 2020·Environmental Science & Technology·Lee F StanishJoseph N Ryan

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Methods Mentioned

BETA
PCR

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