Expanding the "minimalist" small molecule tagging approach to different bioactive compounds

Organic & Biomolecular Chemistry
Wenjie LangJingyan Ge

Abstract

"Minimalist" small molecule tagging (MSMT) is a promising approach that easily converts bioactive compounds into affinity-based probes (AfBPs) for proteomic studies. In this work, seven bioactive compounds targeting diversified protein classes were installed with "minimalist" linkers through common reactions to generate the corresponding AfBPs. These probes were evaluated for cell-based protein profiling and target validation. Among them, the entinostat-derived probe EN and the camptothecin-derived probe CA were further utilized in cellular imaging and SILAC-based large-scale target identification. Our extensive studies suggest that the "minimalist" small molecule tagging approach could be expanded to different classes of bioactive compounds for modification into AfBPs as a dual functional tool for both proteomics and cellular imaging.

References

May 29, 2004·Journal of Molecular Biology·Jill E ChrencikMatthew R Redinbo
Aug 10, 2006·Chemical Reviews·Michael J Evans, Benjamin F Cravatt
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Cleo M Salisbury, Benjamin F Cravatt
Jun 11, 2010·Chemistry & Biology·Yves PommierChristophe Marchand
Jun 23, 2010·Journal of Medicinal Chemistry·Tobias KarlbergHerwig Schüler
Jan 17, 2012·Journal of the American Chemical Society·Haibin ShiShao Q Yao
Apr 17, 2012·Natural Product Reports·Malte GerschStephan A Sieber
May 31, 2012·Angewandte Chemie·Katherine S YangRalph Weissleder
Jul 25, 2012·Nature Reviews. Cancer·Oliver Rath, Frank Kozielski
Nov 13, 2012·Nature Chemical Biology·Ratika KrishnamurtyDustin J Maly
Feb 19, 2013·Angewandte Chemie·Slava ZieglerHerbert Waldmann
Feb 20, 2013·Accounts of Chemical Research·Lin YuanSasa Zhu
Jun 26, 2013·Current Opinion in Chemical Biology·Ying SuShao Q Yao
Jun 7, 2014·Annual Review of Biochemistry·Micah J Niphakis, Benjamin F Cravatt
Jul 19, 2014·Nature Reviews. Drug Discovery·Jörg EderChristian Wiesmann
May 15, 2015·Journal of Medicinal Chemistry·Ute F RöhrigOlivier Michielin
Oct 29, 2015·Chemical Society Reviews·Tim CernakShane W Krska
Mar 5, 2016·Cell Chemical Biology·Bridget K Wagner, Stuart L Schreiber
Apr 6, 2016·Accounts of Chemical Research·Linghui QianShao Q Yao
Sep 16, 2016·Nature Reviews. Genetics·Peter A JonesStephen Baylin
Mar 1, 2017·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Nupur Gupta, Jay B Wish
Jul 8, 2017·Nature Reviews. Drug Discovery·John G MoffatMarco Prunotto
Nov 7, 2017·European Journal of Medicinal Chemistry·Martina FerriEmidio Camaioni
Aug 31, 2018·ACS Central Science·Neal K Devaraj

❮ Previous
Next ❯

Citations

Dec 4, 2019·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takao Yamaguchi
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zetryana Puteri TachrimMakoto Hashimoto

❮ Previous
Next ❯

Methods Mentioned

BETA
affinity purification
fluorescence microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.