Aug 9, 2014

Ion transport mechanisms for smoke inhalation injured airway epithelial barrier

bioRxiv
Xin LiJamie Cate

Abstract

Smoke inhalation injury is the leading cause of death in firefighters and victims. Inhaled hot air and toxic smoke are the predominant hazards to the respiratory epithelium. We aimed to analyze the effects of thermal stress and smoke aldehyde on the permeability of the airway epithelial barrier. Transepithelial resistance (RTE) and the short-circuit currents (ISC) of primary mouse tracheal epithelial (MTE) monolayers were digitized by an Ussing chamber setup. Zonula occludens (ZO)-1 tight junction protein was visualized under confocal microscopy. A cell viability test and fluorescein isothiocyanate-dextran assay were performed. Thermal stress (40 C) decreased RTE in a two-phase manner. Meanwhile, thermal stress increased ISC followed by its decline. Na+ depletion, amiloride (inhibitor for epithelial Na+ channels [ENaCs]), ouabain (blocker for Na+/K+-ATPase) and CFTRinh-172 (blocker of cystic fibrosis transmembrane regulator [CFTR]) altered the responses of RTE and ISC to thermal stress. Steady-state 40 C increased activity of ENaCs, Na+/K+-ATPase, and CFTR. Acrolein, which is one of the main oxidative unsaturated aldehydes in fire smoke, eliminated RTE and ISC. Na+ depletion, amiloride, ouabain, and CFTRinh-172 suppressed acrol...Continue Reading

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Mentioned in this Paper

Metabolic Process, Cellular
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Biochemical Pathway
Saccharomyces cerevisiae allergenic extract
Protoplasm
Yeasts
Cell Wall (Plants)
Xylose Reductase
Cell Wall
Tandem

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