Expansion and activation of natural killer cells from PBMC for immunotherapy of hepatocellular carcinoma

World Journal of Gastroenterology : WJG
Bao-Gang PengMing-De Lu

Abstract

To induce efficient expansion of natural killer (NK) cells from peripheral blood mononuclear cells (PBMCs) using a culture of anchorage-dependent Wilms tumor cell lines, and to provide a reliable supply for adoptive immunotherapy of hepatocellular carcinoma. Culture expansion of NK cells was achieved using PBMCs cultured with Wilms tumor cells. Cytotoxicity was measured using a standard (51)Cr release assay and crystal violet staining technique. The proportions of CD3+, CD4+, CD8+, CD16+, and CD56+ cells were determined by flow cytometry. After PBMCs from healthy donors and hepatocellular carcinoma (HCC) were cultured with irradiated HFWT cells for 10-21 d, CD56+ CD16+ cells shared more than 50% of the cell population, and more than 80% of fresh HFWT cells were killed at an effector/target ratio of 2 over 24 h. NK-enriched lymphocyte population from HCC patients killed HCC-1 and 2 cells with sensitivities comparable to fresh TKB-17RGB cells. HCC cells proliferated 196-fold with the irradiated HFWT cells at 18 d. Stimulation by HFWT cells required intimate cell-cell interaction with PBMC. However, neither the soluble factors released from HFWT cells nor the fixed HFWT cells were effective for NK expansion. The lymphocytes expand...Continue Reading

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Citations

Dec 14, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jinling HuangChen Dong
Mar 22, 2005·Bone Marrow Transplantation·J J Auletta, H M Lazarus
May 12, 2017·Frontiers in Immunology·Markus GranzinEvelyn Ullrich
Jun 23, 2018·Frontiers in Immunology·Thibaut PerchetRachel Golub
Dec 15, 2020·American Journal of Reproductive Immunology : AJRI·Olivia K TravisDenise C Cornelius
Feb 13, 2021·Journal of Immunological Methods·Riya S PatelBiju Parekkadan

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