Expansion of Airway Basal Cells and Generation of Polarized Epithelium

Bio-protocol
Hannah LevardonHongmei Mou

Abstract

Airway basal stem cells are the progenitor cells within the airway that exhibit the capacity to self-renew and give rise to multiple types of differentiated airway epithelial cells. This stem cell-derived epithelium displays organized architecture with functional attributes of the airway mucosa. A protocol has been developed to culture and expand human airway basal stem cells while preserving their stem cell properties and capacity for subsequent mucociliary differentiation. This achievement presents a previously unrealized opportunity to maintain a durable supply of progenitor cells derived from healthy donors to differentiate into human primary airway epithelium for cellular and molecular-based studies. Further, basal stem cells can be harvested from patients with a specific airway disease, such as cystic fibrosis, enabling investigation of potentially altered behavior of disease-specific cells in the appropriate context of the airway mucosa. Here we describe, in detail, a protocol for the serial expansion of airway basal stem cells to enable the generation of nearly unlimited airway basal cells that can be stored and readily available for subsequent culturing and differentiation. In addition, we describe culturing and differ...Continue Reading

References

Oct 26, 2012·Methods in Molecular Biology·Yingjian You, Steven L Brody
Mar 25, 2015·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Lael M YonkerAlessandra Bragonzi
Aug 16, 2017·Scientific Reports·Lael M YonkerBryan P Hurley
Sep 10, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lael M YonkerBryan P Hurley

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Citations

Sep 26, 2019·Scientific Reports·Bryan P HurleyRachel Rosen
Oct 21, 2020·Biochemical Society Transactions·Eamon Dubaissi
Jan 9, 2021·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Nikhil T AwatadeShafagh A Waters
Nov 25, 2020·The American Journal of Pathology·Alexandra McCarronMartin Donnelley
Apr 22, 2021·Cell Reports·Hongmei MouXingbin Ai
Apr 27, 2021·Frontiers in Medicine·Donald R CampbellChelsea M Magin
May 27, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Colleen M BartmanSergio E Chiarella
Aug 31, 2021·Molecular Therapy. Nucleic Acids·Ashley L CooneyPatrick L Sinn

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