PMID: 6974158Feb 1, 1981Paper

Expansion of human lymphocyte populations expressing specific immune reactivities. III. Specific colonies, either cytotoxic or proliferative, obtained from a population of responder cells primed in vitro. Preliminary immunogenetic analysis

Human Immunology
B MalissenC Mawas

Abstract

Human alloreactive cell lines were maintained in culture over prolonged periods of time using conditioned medium. Primed lymphocyte typing reactivity was observed in these T cell lines for only 1 mo, but these T cell lines have remained for more than 7 mo highly and specifically cytotoxic. Using as growth promoter an irradiated autologous feeder consisting of irradiated peripheral blood lymphocytes and the lectin leucoagglutinin, we have derived by limiting dilution cloning of in vitro primed allogeneic combinations, primary colonies (or primary clones) with monofunctional immune reactivities: either cytotoxic (the rarest observed) or PLT reactive (the majority of the colonies). Furthermore, each monofunctional primary colony when tested for PLT or CML reactivity on a panel of unrelated PBL, always showed a restricted specificity when compared to the original primed population. The PLT reactivity of each of the primary clones was short lasting in contrast to their growth potential. The CML reactivity of the primary clones, as for the T cell lines, was long lasting as was their growth potential.

References

Jan 1, 1977·Scandinavian Journal of Immunology·P S CrosierJ Dausset
Jun 1, 1977·European Journal of Immunology·G Dennert, W Raschke
Aug 1, 1979·European Journal of Immunology·H von BoehmerW Haas
Sep 1, 1979·European Journal of Immunology·A A AugustinH Cosenza
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May 1, 1979·European Journal of Immunology·G T Pang, R J Booth
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Citations

Jan 1, 1984·Immunogenetics·E GoulmyJ J van Rood
Feb 1, 1983·Human Immunology·A ZeeviR J Duquesnoy
Sep 1, 1983·Human Immunology·R J Duquesnoy, A Zeevi
Jan 1, 1981·Immunobiology·D J Schendel, R Wank
Oct 26, 2007·Journal of Immunological Methods·Seung-Tae LeeSattva S Neelapu
Apr 26, 2005·Journal of Immunological Methods·Stuart I ManneringLeonard C Harrison
Jul 1, 1989·Scandinavian Journal of Immunology·K E LundinG Gaudernack
Jan 1, 1983·Scandinavian Journal of Immunology·M López-BotetL Moretta
Jul 1, 1981·Tissue Antigens·T KristensenC Mawas
Oct 15, 1982·Journal of Immunological Methods·F A LemonnierF M Kourilsky

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