Expansion of the Chlamydia trachomatis inclusion does not require bacterial replication

International Journal of Medical Microbiology : IJMM
Patrik EngströmSven Bergström

Abstract

Chlamydia trachomatis replication takes place inside of a host cell, exclusively within a vacuole known as the inclusion. During an infection, the inclusion expands to accommodate the increasing numbers of C. trachomatis. However, whether inclusion expansion requires bacterial replication and/or de novo protein synthesis has not been previously investigated in detail. Therefore, using a chemical biology approach, we herein investigated C. trachomatis inclusion expansion under varying conditions in vitro. Under normal cell culture conditions, inclusion expansion correlated with C. trachomatis replication. When bacterial replication was inhibited using KSK120, an inhibitor that targets C. trachomatis glucose metabolism, inclusions expanded even in the absence of bacterial replication. In contrast, when bacterial protein synthesis was inhibited using chloramphenicol, expansion of inclusions was blocked. Together, these data suggest that de novo protein synthesis is necessary, whereas bacterial replication is dispensable for C. trachomatis inclusion expansion.

References

Oct 13, 2000·Molecular Microbiology·E R Iliffe-Lee, G McClarty
Jul 2, 2008·Infection and Immunity·Ine Jorgensen, Raphael H Valdivia
Aug 26, 2009·Communicative & Integrative Biology·Yadunanda Kumar, Raphael H Valdivia
Dec 17, 2009·FEMS Immunology and Medical Microbiology·Patrik EngströmJörgen Johansson
May 28, 2010·The Journal of Infectious Diseases·Catherine L HaggertyRoberta B Ness
Jan 1, 2010·Frontiers in Microbiology·Scot P Ouellette, Rey A Carabeo
Jun 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Bidong D NguyenRaphael H Valdivia
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Anders OmslandTed Hackstadt
Nov 14, 2014·Nature Communications·Erik GonzálezThomas F Meyer

❮ Previous
Next ❯

Citations

Feb 6, 2016·Journal of Medicinal Chemistry·James A D GoodFredrik Almqvist
Apr 7, 2021·Infection and Immunity·Karissa J MuñozChristine Sütterlin
Oct 21, 2017·Journal of Medicinal Chemistry·James A D GoodFredrik Almqvist

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Annales de dermatologie et de vénéréologie
N DupinSection MST de la SFD
BMJ : British Medical Journal
M Griffiths
Zeitschrift für die gesamte Hygiene und ihre Grenzgebiete
M HartmannI Zenner
© 2021 Meta ULC. All rights reserved