Apr 17, 2020

Isoproterenol-induced Cardiac Dysfunction in Male and Female C57Bl/6 Mice

BioRxiv : the Preprint Server for Biology
M. K. O. GrantBeshay Zordoky

Abstract

Sex-related differences in cardiovascular diseases are complex and context-dependent. The objective of this work was to comprehensively determine key sex differences in the response to acute and chronic adrenergic stimulation in C57Bl/6 mice. Cardiac function was assessed by trans-thoracic echocardiography before and after acute adrenergic stimulation (a single sub-cutaneous dose of isoproterenol 10 mg/kg) in male and female C57Bl/6 mice. Thereafter, chronic adrenergic stimulation was achieved by sub-cutaneous injections of isoproterenol 10 mg/kg/day for 14 days in male and female mice. Cardiac function and morphometry were assessed by trans-thoracic echocardiography on the 15th day. Thereafter, the mice were euthanized and the hearts were collected. Histopathological analysis of myocardial tissue was performed after staining with hematoxylin & eosin, trichrome, and MAC-2 antibody. Gene expression of remodeling and fibrotic markers was assessed by real-time PCR. Cardiac function and morphometry were also measured before and after isoproterenol 10 mg/kg/day for 14 days in groups of gonadectomized male and female mice and sham-operated controls. In the current work, there were no statistically significant differences in key echoc...Continue Reading

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Mentioned in this Paper

RNA Polymerase II
Genes
DNA-Directed RNA Polymerase
Endonuclease
MT-TA gene
Triplet Codon-amino Acid Adaptor Activity
Drosophila
Transfer RNA
Cell Growth
Genetic Vectors

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