Expansion of the trans-Golgi network following activated collagen secretion is supported by a coiled-coil microtubule-bundling protein, p180, on the ER

Experimental Cell Research
Tomonori UenoKiyoko Ogawa-Goto

Abstract

A coiled-coil endoplasmic reticulum (ER) protein, p180, was originally reported as a ribosome-binding receptor on the rough ER and is highly expressed in secretory tissues. Recently, we reported new functions of p180 as a microtubule-bundling protein on the ER. Here, we investigated the specific roles of p180 in the Golgi complex organization following stimulated collagen secretion. Targeted depletion of p180 by siRNA transfection caused marked reduction of TGN, while other marker levels for the cis or medial Golgi were not markedly changed. Ascorbate stimulation resulted in trans-Golgi network (TGN) expansion to the periphery in control cells that is characterized by both increased membrane amounts and extended shape. In contrast, loss of p180 resulted in retraction of the TGN regardless of ascorbate stimulation. The TGN developed to the periphery along stabilized microtubule bundles, and overexpression of MTB-1 fragment caused dominant-negative phenotypes. Once disorganized, the retracted TGN did not recover in the absence of p180 despite elevated acetylated tubulin levels. TGN46 and p180 were co-distributed in epithelial basal layer cells of human mucosal and gastrointestinal tissues. Taken together, we propose a novel funct...Continue Reading

References

Sep 30, 1975·Annals of the New York Academy of Sciences·C I Levene, C J Bates
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S MuradS R Pinnell
Jul 1, 1995·The Journal of Cell Biology·E E WankerD I Meyer
Jun 17, 1998·DNA and Cell Biology·R LangleyG W Krissansen
Oct 3, 1998·Trends in Biochemical Sciences·G DrewesE M Mandelkow
Mar 24, 1999·The Journal of Cell Biology·M S LadinskyL A Staehelin
Sep 1, 2001·Cellular and Molecular Life Sciences : CMLS·F GuG Thomas
May 25, 2002·Nature·Charlotte HubbertTso-Pang Yao
Dec 18, 2002·The EMBO Journal·Akihisa MatsuyamaMinoru Yoshida
Mar 15, 2003·The Journal of Biological Chemistry·Mat Leighton, Karl E Kadler
Apr 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Stephen J HaggartyStuart L Schreiber
Aug 14, 2003·Biochimica Et Biophysica Acta·Alison K Gillingham, Sean Munro
Jun 9, 2004·Biochemical and Biophysical Research Communications·Russell J DiefenbachAnthony L Cunningham
Mar 23, 2005·The Journal of Cell Biology·Francis A Barr, Johannes Egerer
Mar 25, 2005·Journal of Cell Science·Elizabeth G Canty, Karl E Kadler
Feb 21, 2006·Nature Cell Biology·Manojkumar A PuthenveeduAdam D Linstedt
Feb 23, 2007·Molecular Biology of the Cell·Pierfrancesco MarraMaria Antonietta De Matteis
Jan 25, 2008·Histochemistry and Cell Biology·Alberto LuiniRoman S Polishchuk
Apr 25, 2008·Molecular Biology of the Cell·Timothy N Feinstein, Adam D Linstedt
Jul 22, 2008·Nature Cell Biology·Teodoro PulvirentiAlberto Luini
Nov 28, 2008·Molecular Biology of the Cell·Payam BenyaminiDavid I Meyer

❮ Previous
Next ❯

Citations

Feb 14, 2014·Molecular Biology of the Cell·Merja JoensuuEija Jokitalo
Dec 14, 2011·Nucleic Acids Research·Tomonori UenoKiyoko Ogawa-Goto
Apr 23, 2013·Biochimica Et Biophysica Acta·Yoshihiro Ishikawa, Hans Peter Bächinger
May 17, 2012·Viruses·Travis R Ruch, Carolyn E Machamer
Apr 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tomonori UenoKiyoko Ogawa-Goto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.