Expansions and contractions in 36-bp minisatellites by gene conversion in yeast.

Genetics
F PâquesJ E Haber

Abstract

The instability of simple tandem repeats, such as human minisatellite loci, has been suggested to arise by gene conversions. In Saccharomyces cerevisiae, a double-strand break (DSB) was created by the HO endonuclease so that DNA polymerases associated with gap repair must traverse an artificial minisatellite of perfect 36-bp repeats or a yeast Y' minisatellite containing diverged 36-bp repeats. Gene conversions are frequently accompanied by changes in repeat number when the template contains perfect repeats. When the ends of the DSB have nonhomologous tails of 47 and 70 nucleotides that must be removed before repair DNA synthesis can begin, 16% of gene conversions had rearrangements, most of which were contractions, almost always in the recipient locus. When efficient removal of nonhomologous tails was prevented in rad1 and msh2 strains, repair was reduced 10-fold, but among survivors there was a 10-fold reduction in contractions. Half the remaining events were expansions. A similar decrease in the contraction rate was observed when the template was modified so that DSB ends were homologous to the template; and here, too, half of the remaining rearrangements were expansions. In this case, efficient repair does not require RAD1 ...Continue Reading

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Citations

Apr 5, 2003·Genomics·Philippe R J Bois
Jun 16, 2004·Nature Reviews. Microbiology·Kevin J VerstrepenGerald R Fink
Aug 12, 2008·Fungal Genetics and Biology : FG & B·Nataliya KucheryavaMatthew D Templeton
Apr 20, 2006·Molecular Biology and Evolution·Suzanne Bowen, Alan E Wheals
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·E KrausJ E Haber
Sep 24, 2005·Molecular Biology and Evolution·Guy-Franck Richard, Bernard Dujon
Jan 11, 2005·Journal of Molecular Evolution·Yuri TrusovVladimir A Berdnikov
Jun 15, 2007·Eukaryotic Cell·Emma LevdanskyNir Osherov
Aug 2, 2003·Molecular Microbiology·Catarina G ResendeMick F Tuite
Sep 19, 2001·Yeast
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Guy-Franck RichardBernard Dujon
Jan 11, 2002·Molecular and Cellular Biology·Peter A JauertDavid T Kirkpatrick
Apr 3, 2010·Nucleic Acids Research·Thomas RollandGuy-Franck Richard
Feb 25, 2011·PLoS Biology·Angela DeemAnna Malkova

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