PMID: 11339297May 8, 2001Paper

Expedient liquid chromatographic method with fluorescence detection for montelukast sodium in micro-samples of plasma

Journal of Chromatography. B, Biomedical Sciences and Applications
Sameer Al-RawithiD Raines

Abstract

This study describes an expedient assay for the analysis of the asthma medication, montelukast sodium (Singulair, MK-0476), in human plasma samples. After a simple extraction of the plasma, the drug and internal standard, quinine bisulfate, were measured by HPLC. The chromatographic system consisted of a single pump, a refrigerated autosampler, a C8 4-microm particle size radial compression cartridge at 40 degrees C and a fluorescence detector with the excitation and emission wavelengths set at 350 and 400 nm, respectively. The mobile phase which was delivered at 1.0 ml/min, was prepared by adding 200 ml of 0.025 M sodium acetate, pH adjusted to 4.0 with acetic acid, to 800 ml of acetonitrile, with 50 microl triethylamine. With a run time of only 10 min per sample, this assay had an overall recovery of >97% with a detection limit of 1 ng/ml. The inter- and intra-run relative standard deviations at 0.05, 0.2 and 1.0 microg/ml were all <9.2%, while the analytical recovery at the same concentrations were within 7.7% of the amount added.

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Citations

Nov 19, 2003·Neuroscience Letters·Shao-gang LuScott Herness
Feb 24, 2010·Indian Journal of Pharmaceutical Sciences·Smita PatilV G Khire
Aug 24, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Robert PappElizabeth Kwong
Nov 23, 2006·Journal of Pharmaceutical and Biomedical Analysis·I Durán MerásD Airado Rodríguez
Mar 5, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A Y WuW Li
Dec 11, 2020·Drug Development and Industrial Pharmacy·Emily Yii Ling WongKok Khiang Peh

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