Expediting Antibody Discovery with a Cell and Bead Multiplexed Competition Assay

SLAS Discovery
Zhaoping Liu, John O'Rourke

Abstract

With advances in molecular engineering and humanization, monoclonal antibodies are one of the fastest-growing classes of biopharmaceuticals. During antibody discovery, antibody from hybridoma or primary B-cell supernatants is screened for the desired binding characteristics, and secondary screens measure antibody function and concentration, identify immunoglobulin G (IgG) isotype, and assess cell health. In order to expedite the antibody discovery process, we developed a high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health. No differences in assay performance were observed between single and multiplex formats. The linear range of the assay was from 0.5 to 50 µg/mL, and washing was not required, decreasing assay time and variability. Slight modifications to the protocol allowed quantification of dilute antibody supernatants (0.1-5 µg/mL). Using hybridoma cultures, we showed that cell viability measurements in the assay did not interfere with the bead-based IgG measurements. The assay described here is a simple mix-and-read, no-dilution screen that can reduce the time to antibody cloning and production. The high-content data can differentiat...Continue Reading

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Citations

Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky

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Methods Mentioned

BETA
antibody display
transgenic
PCR
flow cytometry
ELISA
ELISAs
biolayer interferometry
biosensor
AlphaLISA

Software Mentioned

iQue Screener
iQue Screener PLUS
ForeCyt

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