Expeditious discrimination of four species of the Panax genus using direct infusion-MS/MS combined with multivariate statistical analysis

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Shinae KimSung Won Kwon

Abstract

A practical approach based on direct infusion-MS/MS (DI-MS/MS) was demonstrated for metabolomic classification of four species in the Panax genus. The species Panax ginseng, Panax notoginseng, Panax quinquefolius and Panax vietnamensis were analyzed to develop an efficient tool for authenticating ginseng. Four target ions (m/z 783.5, 945.5, 1107.5 and 1149.2) were selected from LC-MS screening results for DI-MS/MS analysis. The target ions served as classifiers of the four species. As a targeted analysis, DI-MS/MS provided the structural identities of the target ions, clear spectral data and high sensitivity in a shorter time than routine LC-MS analysis. Principal component analysis and partial least squares-discriminant analysis of the DI-MS/MS fingerprinting revealed distinct grouping of the data. The results were validated by cross-validation and a permutation test to examine the utility of the statistical models. The spectral intensities of each species were compared with one another using box plots, which allowed straightforward authentication of the Panax species. The proposed method showed improved efficiency over other current methods for discrimination of large quantities of plant material. Additionally, to the best of...Continue Reading

References

Sep 17, 2002·Chemical Research in Toxicology·Bridgette M Beckwith-HallJeremy K Nicholson
Jun 21, 2003·Clinical Chemistry·Thomas M Annesley
Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nicola Fuzzati
Oct 6, 2006·Biotechnology and Bioengineering·Sandrine MasJens Nielsen
Jan 24, 2007·Rapid Communications in Mass Spectrometry : RCM·Eric C Y ChanHwee-Ling Koh
Aug 10, 2007·Phytochemical Analysis : PCA·Patricia Kaori Soares, Ieda Spacino Scarminio
Nov 13, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Manori J SilvaAntonia M Calafat
Mar 6, 2008·Rapid Communications in Mass Spectrometry : RCM·María J Lerma-GarcíaErnesto F Simó-Alfonso
Apr 12, 2008·BMC Bioinformatics·Maris LapinsJarl E S Wikberg
May 12, 2009·Nucleic Acids Research·Jianguo XiaDavid S Wishart
Jan 25, 2011·Natural Product Reports·Lian-Wen QiChun-Su Yuan
Sep 16, 2011·Journal of Agricultural and Food Chemistry·Nahyun KimDongho Lee
May 18, 2012·Metabolomics : Official Journal of the Metabolomic Society·Ewa SzymańskaJohan A Westerhuis
Apr 5, 2013·Journal of Food Science·Chu ChuLi Liu

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Citations

Mar 14, 2020·Biomedical Chromatography : BMC·Wenping HuangShilin Yang
Feb 21, 2018·Phytochemical Analysis : PCA·Wei WuShuying Liu
Aug 22, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jae Won LeeDae Young Lee
Dec 26, 2018·Journal of Analytical Methods in Chemistry·Yinping LiZhiwei Deng

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